|Recombinant Human HSP27 Protein (Catalog#10351-H08E)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
No cross-reactivity with E.coli cell lysate in ELISA.
WB: 2-10 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HSP27. The detection limit for HSP27 is approximately 0.0195 ng/well.
ELISA(Det): 0.1-1 µg/ml
This antibody will detect Human HSPB1 / HSP 27 in ELISA pair set (Catalog: # SEK10351). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10351-R017).
IP: 4-6 μg/mg of lysate