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HSP27 / HSPB1 Antibody, Mouse MAb

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Human HSP27 Antibody Product Information
Immunogen:Recombinant Human HSP27 Protein (Catalog#10351-H08E)
Clone ID:4E 8H3
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human HSP27 Antibody Usage Guide
Specificity:Human HSP27
No cross-reactivity with E.coli cell lysate in ELISA.
Application:WB, ELISA, IP

WB: 2-10 μg/mL

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HSP27. The detection limit for HSP27 is approximately 0.0195 ng/well.

IP: 4-6 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human HSP27 Antibody WB Application Image
Human HSP27 Antibody IP Application Image
Other HSP27 Antibody Products
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Catalog: 10351-MM01-200
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"