HSF1 Antibody (Antigen Affinity Purified)

HSF1 Antibody ( Antigen Affinity Purified ) Datasheet

HSF1 Antibody Product Information

• Immunogen: Recombinant Human HSF1 protein (Catalog#12245-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human HSF1 (rh HSF1; Catalog#12245-H07E; Q00613-1; Asp 2-Ser 529). HSF1 specific IgG was purified by Human HSF1 affinity chromatography.

HSF1 Antibody Usage Guide

• Specificity: Human HSF1
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human HSF1 in human bladder carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing nucleus staining of transitional epithelium. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of human HSF1 in human bladder with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human HSF1 in human placenta with rabbit polyclonal antibody(1µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HSF1 in WB. Using a DAB detection system, the detection limit for Human HSF1 is approximately 8 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HSF1. The detection limit for Human HSF1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HSF1 Antibody Background

Heat shock factor protein 1, also known as heat shock transcription factor 1, HSF1 and HSTF1, is a cytoplasm and nucleus protein which belongs to theHSF family. HSF1 is the major transcription factor of HSPs (heat shock proteins) in response to various stresses. Wild type HSF1 (heat shock transcriptional factor 1) is normally inactive. HSF1 / HSTF1 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF1 / HSTF1 protects cells and organisms against various types of stress, either by triggering a complex response that promotes cell survival or by triggering cell death when stress-induced alterations cannot be rescued. HSF1 / HSTF1 is the key protein in regulating stress response. It can be activated under heat, oxidative or another stress conditions. Dominant-positive and dominant-negative HSF1 are two types of HSF1 mutants. Both of them gain the DNA binding activity in the absence of stress. In addition, dominant-positive HSF1 acquires transcriptional activity, which dominant-negative HSF1 does not acquire. HSF1 / HSTF1 was also reported to contribute to cell resistance against genotoxic stress, such as that caused by doxorubicin, an anticancer drug in common clinical use.

References

1. Holmberg,C.I. et al., 2000, Cell Stress Chaperones. 5 (3): 219-28.
2. Huang,Y.H. et al., 2007, Sheng Wu Gong Cheng Xue Bao. 23 (6): 971-5.
3. Salmand,P.A. et al.,2008, Biol Reprod. 79 (6): 1092-101.
4. Lee,Y.J. et al., 2008, Cancer Res. 68 (18): 7550-60.
5. Hou,Y. et al., 2009, Mol Biol Rep. 36 (8): 2271-7.