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Human HPRT1 Gene cDNA Clone (full-length ORF Clone)
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Human HPRT1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG12034-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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HPRT1 cDNA Clone Datasheet

 

HPRT1 cDNA Clone Product Information

Gene Bank Ref.ID: NM_000194

cDNA Size:

657

Gene Synonym:

HGPRT

Source: Human

HPRT1 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens hypoxanthine phosphoribosyltransferase 1 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

HPRT1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HPRT1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

HPRT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGACCC GCAGCCCTGG CGTCGTGATT AGTGATGATG AACCAGGTTA TGACCTTGAT
61 TTATTTTGCA TACCTAATCA TTATGCTGAG GATTTGGAAA GGGTGTTTAT TCCTCATGGA
121 CTAATTATGG ACAGGACTGA ACGTCTTGCT CGAGATGTGA TGAAGGAGAT GGGAGGCCAT
181 CACATTGTAG CCCTCTGTGT GCTCAAGGGG GGCTATAAAT TCTTTGCTGA CCTGCTGGAT
241 TACATCAAAG CACTGAATAG AAATAGTGAT AGATCCATTC CTATGACTGT AGATTTTATC
301 AGACTGAAGA GCTATTGTAA TGACCAGTCA ACAGGGGACA TAAAAGTAAT TGGTGGAGAT
361 GATCTCTCAA CTTTAACTGG AAAGAATGTC TTGATTGTGG AAGATATAAT TGACACTGGC
421 AAAACAATGC AGACTTTGCT TTCCTTGGTC AGGCAGTATA ATCCAAAGAT GGTCAAGGTC
481 GCAAGCTTGC TGGTGAAAAG GACCCCACGA AGTGTTGGAT ATAAGCCAGA CTTTGTTGGA
541 TTTGAAATTC CAGACAAGTT TGTTGTAGGA TATGCCCTTG ACTATAATGA ATACTTCAGG
601 GATTTGAATC ATGTTTGTGT CATTAGTGAA ACTGGAAAAG CAAAATACAA AGCCTAA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"