Anti-15-PGDH Antibody

Cat: 50531-RP02
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Anti-15-PGDH Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-15-PGDH Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse 15-PGDH
Immunogen
Recombinant Mouse HPGD / 15-PGDH protein (Catalog#50531-M08E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse HPGD / 15-PGDH (rM HPGD / 15-PGDH; Catalog#50531-M08E; Q8VCC1; Met 1-Ser 269). HPGD / 15-PGDH specific IgG was purified by Mouse HPGD / 15-PGDH affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-15-PGDH Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-15-PGDH Antibody (Rabbit Polyclonal antibody) Images
Mouse HPGD/15-PGDH Immunoprecipitation(IP) 8241

Mouse HPGD was immunoprecipitated using:

Lane A:0.5 mg Caco-2 Whole Cell Lysate

2 µL anti-Mouse HPGD rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse HPGD rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 29 kDa

Mouse HPGD/15-PGDH Western blot (WB) 13685

Anti-HPGD rabbit polyclonal antibody at 1:500 dilution

Lane A: Lovo Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:34 kDa

(We are unsure as to the identity of these extra bands.)

Anti-15-PGDH Antibody Alternative Names
Anti-15-PGDH Antibody;Anti-AV026552 Antibody
15-PGDH Background Information

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.

Full Name
hydroxyprostaglandin dehydrogenase 15-(NAD)
References
  • Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33.
  • McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41.
  • Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73.
  • Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-15-PGDH Antibody Rabbit Monoclonal WB,IP 50531-R101
    Anti-15-PGDH Antibody Rabbit Polyclonal ELISA 50531-RP01
    Anti-15-PGDH Antibody Rabbit Monoclonal ELISA 50531-R121
    Anti-15-PGDH Antibody Rabbit Monoclonal FCM,ICC/IF,IF 11205-R027
    Anti-15-PGDH Antibody Rabbit Monoclonal FCM,ICC/IF,IF 11205-R016
    Anti-15-PGDH Antibody Rabbit Monoclonal ELISA,ELISA(Det) 11205-R025
    Anti-15-PGDH Antibody Mouse Monoclonal ELISA 11205-MM01
    Anti-15-PGDH Antibody (FITC) Rabbit Monoclonal FCM 11205-R027-F
    Anti-15-PGDH Antibody Rabbit Monoclonal ELISA(Cap) 11205-R004
    Anti-15-PGDH Antibody (FITC) Rabbit Monoclonal FCM 11205-R016-F
    Anti-15-PGDH Antibody Mouse Monoclonal WB,ELISA 11205-MM02
    Anti-15-PGDH Antibody Rabbit Polyclonal ELISA 11205-RP01
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