Anti-15-PGDH Antibody

Cat: 11205-R027
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Anti-15-PGDH Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-15-PGDH Antibody
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human 15-PGDH
Immunogen
Recombinant Human HPGD / 15-PGDH protein (Catalog#11205-H08E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human HPGD / 15-PGDH (rh HPGD / 15-PGDH; Catalog#11205-H08E; NP_000851.2; Met1-Gln266).
Source
Monoclonal Rabbit IgG Clone #027
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-15-PGDH Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-15-PGDH Antibody (Rabbit Monoclonal antibody) Images
Human HPGD/15-PGDH Immunofluorescence(IF) 2916
Immunofluorescence staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HPGD monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human HPGD/15-PGDH Flow Cytometry (FC) 5159
Flow cytometric analysis of Human HPGD expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-15-PGDH Antibody Alternative Names
Anti-15-PGDH Antibody;Anti-PGDH Antibody;Anti-PGDH1 Antibody;Anti-PHOAR1 Antibody;Anti-SDR36C1 Antibody
15-PGDH Background Information

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.

Full Name
hydroxyprostaglandin dehydrogenase 15-(NAD)
References
  • Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33.
  • McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41.
  • Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73.
  • Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-15-PGDH Antibody Rabbit Monoclonal WB,IP 50531-R101
    Anti-15-PGDH Antibody Rabbit Polyclonal ELISA 50531-RP01
    Anti-15-PGDH Antibody Rabbit Monoclonal ELISA 50531-R121
    Anti-15-PGDH Antibody Rabbit Polyclonal WB,ELISA,IP 50531-RP02
    Anti-15-PGDH Antibody Rabbit Monoclonal FCM,ICC/IF,IF 11205-R016
    Anti-15-PGDH Antibody Rabbit Monoclonal ELISA,ELISA(Det) 11205-R025
    Anti-15-PGDH Antibody Mouse Monoclonal ELISA 11205-MM01
    Anti-15-PGDH Antibody (FITC) Rabbit Monoclonal FCM 11205-R027-F
    Anti-15-PGDH Antibody Rabbit Monoclonal ELISA(Cap) 11205-R004
    Anti-15-PGDH Antibody (FITC) Rabbit Monoclonal FCM 11205-R016-F
    Anti-15-PGDH Antibody Mouse Monoclonal WB,ELISA 11205-MM02
    Anti-15-PGDH Antibody Rabbit Polyclonal ELISA 11205-RP01
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