HOXA1 Protein (His Tag)

Homeobox A1 Protein Datasheet

HOXA1 Protein Product Information

• Synonym: BSAS, HOX1, HOX1F, MGC45232
• Protein Construction: A DNA sequence encoding the mature form of human HOXA1 isoform 3 (P49639-1) (Met 1- His 335) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

HOXA1 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human HOXA1 consisting of 346 amino acids and has a calculated molecular mass of 38 KDa. The apparent molecular mass of the protein is approximately 42 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 30%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: HOXA1 protein

HOXA1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

HOXA1 Protein Description

Homeobox protein Hox-A1 is a transcription factor encoded by HOXA1 gene. These Homeobox genes are essential metazoan genes as they determine the identity of embryonic regions along the anterio-posterior axis. The homeobox protein Hox-A1 may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Early in its development, the vertebrate hindbrain is transiently subdivided into a series of compartments called rhombomeres. Genes have been identified whose expression patterns distinguish these cellular compartments. Two of these genes, Hoxa1 and Hoxa2, have been shown to be required for proper patterning of the early mouse hindbrain and the associated neural crest. It has been detected HOXA1 expression in a variety of human breast cancer lesions, suggesting that HOXA1 may be required for the establishment of breast cancer cells phenotype.

References

1. Barrow JR. et al., 2000, Development. 127: 933-44.
2. Hong YS. et al., 1995, Gene. 159 (2): 209-14.
3. Gavalas A. et al., 2001, Development. 128: 3017-27.