Anti-HNMT Antibody

Cat: 13096-RP02
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Anti-HNMT Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-HNMT Antibody
Validated applications
Species reactivity
Reacts with: Human
Human HNMT
Recombinant Human HNMT protein (Catalog#13096-H09E)
Produced in rabbits immunized with purified, recombinant Human HNMT (rh HNMT; Catalog#13096-H09E; AAH20677.1; Met 1-Ala 292). HNMT specific IgG was purified by Human HNMT affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HNMT Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HNMT Antibody (Rabbit Polyclonal antibody) Images
Human HNMT Western blot (WB) 13526

Anti-HNMT rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

Human HNMT Immunoprecipitation(IP) 17707

HNMT was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-HNMT rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HNMT rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 33 kDa

Anti-HNMT Antibody Alternative Names
Anti-HMT Antibody;Anti-HNMT-S1 Antibody;Anti-HNMT-S2 Antibody
HNMT Background Information
Full Name
histamine N-methyltransferase
  • Yan L., et al., 2000, Pharmacogenetics 10:261-266.
  • Horton J.R., et al., 2001, Structure 9:837-849.
  • Hillier L.W., et al., 2005, Nature 434:724-731.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-HNMT Antibody Rabbit Monoclonal WB,ELISA,IP 13096-R034
    Anti-HNMT Antibody Rabbit Polyclonal WB,ELISA 13096-RP01
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