Anti-HNMT Antibody

Cat: 13096-RP02
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Anti-HNMT Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-HNMT Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human HNMT
Immunogen
Recombinant Human HNMT protein (Catalog#13096-H09E)
Preparation
Produced in rabbits immunized with purified, recombinant Human HNMT (rh HNMT; Catalog#13096-H09E; AAH20677.1; Met 1-Ala 292). HNMT specific IgG was purified by Human HNMT affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HNMT Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HNMT Antibody (Rabbit Polyclonal antibody) Images
Human HNMT Western blot (WB) 13526

Anti-HNMT rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

Human HNMT Immunoprecipitation(IP) 17707

HNMT was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-HNMT rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HNMT rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 33 kDa

Anti-HNMT Antibody Alternative Names
Anti-HMT Antibody;Anti-HNMT-S1 Antibody;Anti-HNMT-S2 Antibody
HNMT Background Information
Full Name
histamine N-methyltransferase
References
  • Yan L., et al., 2000, Pharmacogenetics 10:261-266.
  • Horton J.R., et al., 2001, Structure 9:837-849.
  • Hillier L.W., et al., 2005, Nature 434:724-731.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-HNMT Antibody Rabbit Monoclonal WB,ELISA,IP 13096-R034
    Anti-HNMT Antibody Rabbit Polyclonal WB,ELISA 13096-RP01
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