Mouse HMMR Gene cDNA Clone / ORF Clone

HMMR cDNA Clone Datasheet

HMMR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013552.2
• cDNA Size: 2385
• Gene Synonym: CD168; Rhamm; AA386826; Hmmr
• Source: Mouse

HMMR cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus hyaluronan mediated motility receptor (RHAMM) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 212 A/C resulting in the amino acid Lys substitution by Thr.

HMMR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HMMR cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HMMR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCTTTC CTAAGGCGCC CCTGAAGAGA TTCAATGACC CTTCGGGTTG TGCTCCATCT
61       CCGGGTGCTT ATGATGTTAA AACTTCAGAA GCAACTAAAG GACCAGTGTC TTTTCAGAAA
121      TCACAAAGAT TTAAAAACCA AAGAGAGTCT CAACAAAATC TTAACATTGA CAAAGATACA
181      ACCTTGCTTG CTTCGGCTAA AAAAGCAAAG ACGTCTGTGT CAAAGAAGGA CTCTCAGAAG
241      AATGATAAAG ATGTGAAGAG ATTAGAAAAA GAGATTCGCG CTCTTTTGCA AGAGCGAGGG
301      ACTCAGGACA AACGGATCCA GGACATGGAA TCTGAATTGG AGAAGACAGA AGCAAAGCTC
361      AATGCAGCAG TCAGAGAGAA AACATCTCTC TCTGCGAGTA ATGCTTCACT GGAAAAACGG
421      CTTACTGAAT TAACCAGAGC CAACGAGCTA CTAAAGGCTA AGTTTTCTGA AGATGGTCAC
481      CAAAAGAATA TGAGAGCTCT AAGCCTGGAA TTGATGAAAC TCAGAAATAA GAGAGAGACA
541      AAGATGAGGA GTATGATGGT CAAACAGGAA GGCATGGAGC TGAAGCTGCA GGCCACTCAG
601      AAGGACCTCA CGGAGTCTAA GGGAAAAATA GTCCAGCTGG AGGGAAAGCT TGTTTCAATA
661      GAGAAAGAAA AGATCGATGA AAAATGTGAA ACAGAAAAAC TCTTAGAATA CATCCAAGAA
721      ATTAGCTGTG CATCTGATCA AGTGGAAAAA TGCAAAGTAG ATATTGCCCA GTTAGAAGAA
781      GATTTGAAAG AGAAGGATCG TGAGATTTTA AGTCTTAAGC AGTCTCTTGA GGAAAACATT
841      ACATTTTCTA AGCAAATAGA AGACCTGACT GTTAAATGCC AGCTACTTGA AACAGAAAGA
901      GACAACCTTG TCAGCAAGGA TAGAGAAAGG GCTGAAACTC TCAGTGCTGA GATGCAGATC
961      CTGACAGAGA GGCTGGCTCT GGAAAGGCAA GAATATGAAA AGCTGCAACA AAAAGAATTG
1021     CAAAGCCAGT CACTTCTGCA GCAAGAGAAG GAACTGTCTG CTCGTCTGCA GCAGCAGCTC
1081     TGCTCTTTCC AAGAGGAAAT GACTTCTGAG AAGAACGTCT TTAAAGAAGA GCTAAAGCTC
1141     GCCCTGGCTG AGTTGGATGC GGTCCAGCAG AAGGAGGAGC AGAGTGAAAG GCTGGTTAAA
1201     CAGCTGGAAG AGGAAACGAA GTCAACTGCA GAACAACTGA CGCGGCTGGA CAACCTGCTG
1261     AGAGAGAAAG AAGTTGAACT GGAGAAACAT ATTGCTGCTC ACGCCCAAGC CATCTTGATT
1321     GCACAAGAGA AGTATAATGA CACAGCACAG AGTCTGAGGG ACGTCACTGC TCAGTTGGAA
1381     AGTGTGCAAG AGAAGTATAA TGACACAGCA CAGAGTCTGA GGGACGTCAC TGCTCAGTTG
1441     GAAAGTGAGC AAGAGAAGTA CAATGACACA GCACAGAGTC TGAGGGACGT CACTGCTCAG
1501     TTGGAAAGTG AGCAAGAGAA GTACAATGAC ACAGCACAGA GTCTGAGGGA CGTCACTGCT
1561     CAGTTGGAAA GTGTGCAAGA GAAGTACAAT GACACAGCAC AGAGTCTGAG GGACGTCACT
1621     GCTCAGTTGG AAAGCTATAA GTCATCAACA CTTAAAGAAA TAGAAGATCT TAAACTGGAG
1681     AATTTGACTC TACAAGAAAA AGTAGCTATG GCTGAAAAAA GTGTAGAAGA TGTTCAACAG
1741     CAGATATTGA CAGCTGAGAG CACAAATCAA GAATATGCAA GGATGGTTCA AGATTTGCAG
1801     AACAGATCAA CCTTAAAAGA AGAAGAAATT AAAGAAATCA CATCTTCATT TCTTGAGAAA
1861     ATAACTGATT TGAAAAATCA ACTCAGACAA CAAGATGAAG ACTTTAGGAA GCAGCTGGAA
1921     GAGAAAGGAA AAAGAACAGC AGAGAAAGAA AATGTAATGA CAGAATTAAC CATGGAAATT
1981     AATAAATGGC GTCTCCTATA TGAAGAACTA TATGAAAAAA CTAAACCTTT TCAGCAACAA
2041     CTGGATGCCT TTGAAGCCGA GAAACAGGCA TTGTTGAATG AACATGGTGC AACTCAGGAG
2101     CAGCTAAATA AAATCAGAGA CTCCTATGCA CAGCTACTTG GTCACCAGAA CCTAAAGCAA
2161     AAAATCAAAC ATGTTGTGAA ATTGAAAGAT GAAAATAGCC AACTCAAATC GGAGGTGTCA
2221     AAACTCCGAT CTCAGCTTGT TAAAAGGAAA CAAAATGAGC TCAGACTTCA GGGAGAATTA
2281     GATAAAGCTC TGGGCATCAG ACACTTTGAC CCTTCCAAGG CTTTTTGTCA TGCATCTAAG
2341     GAGAATTTTA CTCCATTAAA AGAAGGCAAC CCAAACTGCT GCTGA

 

Quality Control:cDNA sequencing results is available upon request.