Mouse HMGB1 Gene cDNA Clone / ORF Clone

HMGB1 cDNA Clone Datasheet

HMGB1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010439.3
• cDNA Size: 648
• Gene Synonym: DEF; p30; Hmg1; HMG-1; SBP-1; MGC103168; MGC103169; MGC117896; MGC117897; amphoterin; Hmgb1
• Source: Mouse

HMGB1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus high mobility group box 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations: 21 A/G and 123 C/T not causing the amino acid variation.

HMGB1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HMGB1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HMGB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CCTCATATGC ATTCTTTGTG
61       CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG
121      TTTTCCAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAAAA GGGGAAATTT
181      GAAGATATGG CAAAGGCTGA CAAGGCTCGT TATGAAAGAG AAATGAAAAC CTACATCCCC
241      CCCAAAGGGG AGACCAAAAA GAAGTTCAAG GACCCCAATG CACCCAAGAG GCCTCCTTCG
301      GCCTTCTTCT TGTTCTGTTC TGAGTACCGC CCCAAAATCA AAGGCGAGCA TCCTGGCTTA
361      TCCATTGGTG ATGTTGCAAA GAAACTAGGA GAGATGTGGA ACAACACTGC AGCAGATGAC
421      AAGCAGCCCT ATGAGAAGAA AGCTGCCAAG CTGAAGGAGA AGTATGAGAA GGATATTGCT
481      GCCTACAGAG CTAAAGGAAA ACCTGATGCA GCGAAAAAGG GGGTGGTCAA GGCTGAAAAG
541      AGCAAGAAAA AGAAGGAAGA GGAAGATGAT GAGGAGGATG AAGAGGATGA GGAAGAGGAG
601      GAAGAAGAGG AAGACGAAGA TGAAGAAGAA GATGATGATG ATGAATAA

 

Quality Control:cDNA sequencing results is available upon request.