Human HLA-E Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13375-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-E cDNA Clone Datasheet

HLA-E cDNA Clone Product Information

Gene Bank Ref.ID:	BC002578
cDNA Size:	1077
Gene Synonym:	MHC; QA1; EA1.2; EA2.1; HLA-6.2; HLA-E
Source:	Human

HLA-E cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class I, E DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-E cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-E cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTAGATG GAACCCTCCT TTTACTCCTC TCGGAGGCCC TGGCCCTTAC CCAGACCTGG
61       GCGGGCTCCC ACTCCTTGAA GTATTTCCAC ACTTCCGTGT CCCGGCCCGG CCGCGGGGAG
121      CCCCGCTTCA TCTCTGTGGG CTACGTGGAC GACACCCAGT TCGTGCGCTT CGACAACGAC
181      GCCGCGAGTC CGAGGATGGT GCCGCGGGCG CCGTGGATGG AGCAGGAGGG GTCAGAGTAT
241      TGGGACCGGG AGACACGGAG CGCCAGGGAC ACCGCACAGA TTTTCCGAGT GAACCTGCGG
301      ACGCTGCGCG GCTACTACAA TCAGAGCGAG GCCGGGTCTC ACACCCTGCA GTGGATGCAT
361      GGCTGCGAGC TGGGGCCCGA CAGGCGCTTC CTCCGCGGGT ATGAACAGTT CGCCTACGAC
421      GGCAAGGATT ATCTCACCCT GAATGAGGAC CTGCGCTCCT GGACCGCGGT GGACACGGCG
481      GCTCAGATCT CCGAGCAAAA GTCAAATGAT GCCTCTGAGG CGGAGCACCA GAGAGCCTAC
541      CTGGAAGACA CATGCGTGGA GTGGCTCCAC AAATACCTGG AGAAGGGGAA GGAGACGCTG
601      CTTCACCTGG AGCCCCCAAA GACACACGTG ACTCACCACC CCATCTCTGA CCATGAGGCC
661      ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGCGGAGA TCACACTGAC CTGGCAGCAG
721      GATGGGGAGG GCCATACCCA GGACACGGAG CTCGTGGAGA CCAGGCCTGC AGGGGATGGA
781      ACCTTCCAGA AGTGGGCAGC TGTGGTGGTG CCTTCTGGAG AGGAGCAGAG ATACACGTGC
841      CATGTGCAGC ATGAGGGGCT ACCCGAGCCC GTCACCCTGA GATGGAAGCC GGCTTCCCAG
901      CCCACCATCC CCATCGTGGG CATCATTGCT GGCCTGGTTC TCCTTGGATC TGTGGTCTCT
961      GGAGCTGTGG TTGCTGCTGT GATATGGAGG AAGAAGAGCT CAGGTGGAAA AGGAGGGAGC
1021     TACTCTAAGG CTGAGTGGAG CGACAGTGCC CAGGGGTCTG AGTCTCACAG CTTGTAA

Quality Control:cDNA sequencing results is available upon request.