Human HLA-DRB5 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13255-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-DRB5 cDNA Clone Datasheet

HLA-DRB5 cDNA Clone Product Information

Gene Bank Ref.ID:	BC009234
cDNA Size:	801
Gene Synonym:	HLA-DRB; HLA-DRB5
Source:	Human

HLA-DRB5 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class II, DR bet DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-DRB5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-DRB5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-DRB5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTGTGTC TGAAGCTCCC TGGAGGTTCC TACATGGCAA AGCTGACAGT GACACTGATG
61       GTGCTGAGCT CCCCACTGGC TTTGGCTGGG GACACCCGAC CACGTTTCTT GCAGCAGGAT
121      AAGTATGAGT GTCATTTCTT CAACGGGACG GAGCGGGTGC GGTTCCTGCA CAGAGACATC
181      TATAACCAAG AGGAGGACTT GCGCTTCGAC AGCGACGTGG GGGAGTACCG GGCGGTGACG
241      GAGCTGGGGC GGCCTGACGC TGAGTACTGG AACAGCCAGA AGGACTTCCT GGAAGACAGG
301      CGCGCCGCGG TGGACACCTA CTGCAGACAC AACTACGGGG TTGGTGAGAG CTTCACAGTG
361      CAGCGGCGAG TTGAGCCTAA GGTGACTGTG TATCCTGCAA GGACCCAGAC CCTGCAGCAC
421      CACAACCTCC TGGTCTGCTC TGTGAATGGT TTCTATCCAG GCAGCATTGA AGTCAGGTGG
481      TTCCGGAACA GCCAGGAAGA GAAGGCTGGG GTGGTGTCCA CAGGCCTGAT TCAGAATGGA
541      GACTGGACCT TCCAGACCCT GGTGATGCTG GAAACAGTTC CTCGAAGTGG AGAGGTTTAC
601      ACCTGCCAAG TGGAGCACCC AAGCGTGACG AGCCCTCTCA CAGTGGAATG GAGAGCACAG
661      TCTGAATCTG CACAGAGCAA GATGCTGAGT GGAGTCGGGG GCTTTGTGCT GGGCCTGCTC
721      TTCCTTGGGG CCGGGCTATT CATCTACTTC AAGAATCAGA AAGGGCACTC TGGACTTCAC
781      CCAACAGGAC TCGTGAGCTG A

Quality Control:cDNA sequencing results is available upon request.