Human HLA-DQB1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13322-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-DQB1 cDNA Clone Datasheet

HLA-DQB1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC012106
cDNA Size:	786
Gene Synonym:	IDDM1; CELIAC1; HLA-DQB; HLA-DQB1
Source:	Human

HLA-DQB1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class II, DQ bet DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-DQB1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-DQB1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-DQB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTTGGA AGAAGGCTTT GCGGATCCCT GGAGGCCTTC GGGTAGCAAC TGTGACCTTG
61       ATGCTGGCGA TGCTGAGCAC CCCGGTGGCT GAGGGCAGAG ACTCTCCCGA GGATTTCGTG
121      TACCAGTTTA AGGGCATGTG CTACTTCACC AACGGGACGG AGCGCGTGCG TCTTGTGACC
181      AGATACATCT ATAACCGAGA GGAGTACGCA CGCTTCGACA GCGACGTGGG GGTGTATCGG
241      GCGGTGACGC CGCTGGGGCC GCCTGCCGCC GAGTACTGGA ACAGCCAGAA GGAAGTCCTG
301      GAGAGGACCC GGGCGGAGTT GGACACGGTG TGCAGACACA ACTACCAGTT GGAGCTCCGC
361      ACGACCTTGC AGCGGCGAGT GGAGCCCACA GTGACCATCT CCCCATCCAG GACAGAGGCC
421      CTCAACCACC ACAACCTGCT GGTCTGCTCA GTGACAGATT TCTATCCAGC CCAGATCAAA
481      GTCCGGTGGT TTCGGAATGA CCAGGAGGAG ACAACTGGCG TTGTGTCCAC CCCCCTTATT
541      AGGAACGGTG ACTGGACCTT CCAGATCCTG GTGATGCTGG AAATGACTCC CCAGCGTGGA
601      GACGTCTACA CCTGCCACGT GGAGCACCCC AGCCTCCAGA ACCCCATCAT CGTGGAGTGG
661      CGGGCTCAGT CTGAATCTGC CCAGAGCAAG ATGCTGAGTG GCATTGGAGG CTTCGTGCTG
721      GGGCTGATCT TCCTCGGGCT GGGCCTTATT ATCCATCACA GGAGTCAGAA AGGGCTCCTG
781      CACTGA

Quality Control:cDNA sequencing results is available upon request.