Human HLA-DQA1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13466-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-DQA1 cDNA Clone Datasheet

HLA-DQA1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC008585
cDNA Size:	765
Gene Synonym:	CD; GSE; DQ-A1; CELIAC1; HLA-DQA; HLA-DQA1
Source:	Human

HLA-DQA1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class II, DQ alp DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-DQA1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-DQA1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-DQA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCCTAA ACAAAGCTCT GATGCTGGGG GCCCTTGCCC TGACCACCGT GATGAGCCCC
61       TGTGGAGGTG AAGACATTGT GGCTGACCAC GTCGCCTCTT ATGGTGTAAA CTTGTACCAG
121      TCTTACGGTC CCTCTGGCCA GTACACCCAT GAATTTGATG GAGATGAGCA GTTCTACGTG
181      GACCTGGGGA GGAAGGAGAC TGTCTGGTGT TTGCCTGTTC TCAGACAATT TAGATTTGAC
241      CCGCAATTTG CACTGACAAA CATCGCTGTC CTAAAACATA ACTTGAACAG TCTGATTAAA
301      CGCTCCAACT CTACCGCTGC TACCAATGAG GTTCCTGAGG TCACAGTGTT TTCCAAGTCT
361      CCCGTGACAC TGGGTCAGCC CAACATCCTC ATCTGTCTTG TGGACAACAT CTTTCCTCCT
421      GTGGTCAACA TCACATGGCT GAGCAATGGG CACTCAGTCA CAGAAGGTGT TTCTGAGACC
481      AGCTTCCTCT CCAAGAGTGA TCATTCCTTC TTCAAGATCA GTTACCTCAC CCTCCTCCCT
541      TCTGCTGAGG AGAGTTATGA CTGCAAGGTG GAGCACTGGG GCCTGGACAA GCCTCTTCTG
601      AAACACTGGG AGCCTGAGAT TCCAGCCCCT ATGTCAGAGC TCACAGAGAC TGTGGTCTGC
661      GCCCTGGGAT TGTCTGTGGG CCTCGTGGGC ATTGTGGTGG GCACTGTCTT CATCATCCGA
721      GGCCTGCGTT CAGTTGGTGC TTCCAGACAC CAAGGGCCCT TGTGA

Quality Control:cDNA sequencing results is available upon request.