Human HLA-DMB Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13254-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-DMB cDNA Clone Datasheet

HLA-DMB cDNA Clone Product Information

Gene Bank Ref.ID:	BC027175
cDNA Size:	792
Gene Synonym:	RING7; D6S221E; HLA-DMB
Source:	Human

HLA-DMB cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class II, DM bet DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-DMB cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-DMB cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-DMB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCACAT TCCTGCCGCT GCTGCTGGGG CTCAGCCTGG GCTGCACAGG AGCAGGTGGC
61       TTCGTGGCCC ATGTGGAAAG CACCTGTCTG TTGGATGATG CTGGGACTCC AAAGGATTTC
121      ACATACTGCA TCTCCTTCAA CAAGGATCTG CTGACCTGCT GGGATCCAGA GGAGAATAAG
181      ATGGCCCCTT GCGAATTTGG GGTGCTGAAT AGCTTGGCGA ATGTCCTCTC ACAGCACCTC
241      AACCAAAAAG ACACCCTGAT GCAGCGCTTG CGCAATGGGC TTCAGAATTG TGCCACACAC
301      ACCCAGCCCT TCTGGGGATC ACTGACCAAC AGGACACGGC CACCATCTGT GCAAGTAGCC
361      AAAACCACTC CTTTTAACAC GAGGGAGCCT GTGATGCTGG CCTGCTATGT GTGGGGCTTC
421      TATCCAGCAG AAGTGACTAT CACGTGGAGG AAGAACGGGA AGCTTGTCAT GCCTCACAGC
481      AGTGCGCACA AGACTGCCCA GCCCAATGGA GACTGGACAT ACCAGACCCT CTCCCATTTA
541      GCCTTAACCC CCTCTTACGG GGACACTTAC ACCTGTGTGG TAGAGCACAT TGGGGCTCCT
601      GAGCCCATCC TTCGGGACTG GACACCTGGG CTGTCCCCCA TGCAGACCCT GAAGGTTTCT
661      GTGTCTGCAG TGACTCTGGG CCTGGGCCTC ATCATCTTCT CTCTTGGTGT GATCAGCTGG
721      CGGAGAGCTG GCCACTCTAG TTACACTCCT CTTCCTGGGT CCAATTATTC AGAAGGATGG
781      CACATTTCCT AG

Quality Control:cDNA sequencing results is available upon request.