Human HLA-C Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13264-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-C cDNA Clone Datasheet

HLA-C cDNA Clone Product Information

Gene Bank Ref.ID:	BC008457
cDNA Size:	1119
Gene Synonym:	HLC-C; D6S204; PSORS1; HLA-JY3; HLA-C
Source:	Human

HLA-C cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class I, C DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-C cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-C cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-C cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGGTCA TGGCGCCCCG AACCCTCATC CTGCTGCTCT CGGGAGCCCT GGCCCTGACC
61       GAGACCTGGG CCGGCTCCCA CTCCATGAGG TATTTCTACA CCGCTGTGTC CCGGCCCGGC
121      CGCGGGGAGC CCCACTTCAT CGCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC
181      GACAGCGACG CCGCGAGTCC GAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG
241      CCGGAGTATT GGGACCGGGA GACACAGAAG TACAAGCGCC AGGCACAGAC TGACCGAGTG
301      AGCCTGCGGA ACCTGCGCGG CTACTACAAC CAGAGCGAGG CCAGGTCTCA CATCATCCAG
361      AGGATGTATG GCTGCGACGT GGGGCCCGAC GGGCGCCTCC TCCGCGGGTA TGACCAGTAC
421      GCCTACGACG GCAAGGATTA CATCGCCCTG AACGAGGATC TGCGCTCCTG GACCGCCGCG
481      GACACGGCGG CTCAGATCAC CCAGCGCAAG TGGGAGGCGG CCCGTGAGGC GGAGCAGCTG
541      AGAGCCTACC TGGAGGGCCT GTGCGTGGAG TGGCTCCGCA GATACCTGAA GAATGGGAAG
601      GAGACGCTGC AGCGCGCGGA ACACCCAAAG ACACACGTGA CCCACCATCC CGTCTCTGAC
661      CATGAGGCCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGCGGAGAT CACACTGACC
721      TGGCAGTGGG ATGGGGAGGA CCAAACTCAG GACACTGAGC TTGTGGAGAC CAGGCCAGCA
781      GGAGATGGAA CCTTCCAGAA GTGGGCAGCT GTGGTGGTGC CTTCTGGAGA AGAGCAGAGA
841      TACACGTGCC ATGTGCAGCA CGAGGGGCTG CCGGAGCCCC TCACCCTGAG ATGGGAGCCG
901      TCTTCCCAGC CCACCATCCC CATCGTGGGC ATCGTTGCTG GCCTGGCTGT CCTGGCTGTC
961      CTAGCTGTCC TAGGAGCTGT GGTGGCTGTT GTGATGTGTA GGAGGAAGAG CTCAGGGCAT
1021     TTTCTTCCCA CAGGTGGAAA AGGAGGGAGC TGCTCTCAGG CTGCGTCCAG CAACAGTGCC
1081     CAGGGCTCTG ATGAGTCTCT CATCGCTTGT AAAGCCTGA

Quality Control:cDNA sequencing results is available upon request.