Human HLA-A Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13263-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HLA-A cDNA Clone Datasheet

HLA-A cDNA Clone Product Information

Gene Bank Ref.ID:	BC019236
cDNA Size:	1098
Gene Synonym:	HLAA; HLA-A
Source:	Human

HLA-A cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens major histocompatibility complex, class I, A DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

HLA-A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HLA-A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HLA-A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGTCA TGGCGCCCCG AACCCTCGTC CTGCTACTCT CGGGGGCTCT GGCCCTGACC
61       CAGACCTGGG CGGGCTCTCA CTCCATGAGG TATTTCTTCA CATCCGTGTC CCGGCCCGGC
121      CGCGGGGAGC CCCGCTTCAT CGCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC
181      GACAGCGACG CCGCGAGCCA GAGGATGGAG CCGCGGGCGC CGTGGATAGA GCAGGAGGGT
241      CCGGAGTATT GGGACGGGGA GACACGGAAA GTGAAGGCCC ACTCACAGAC TCACCGAGTG
301      GACCTGGGGA CCCTGCGCGG CTACTACAAC CAGAGCGAGG CCGGTTCTCA CACCGTCCAG
361      AGGATGTATG GCTGCGACGT GGGGTCGGAC TGGCGCTTCC TCCGCGGGTA CCACCAGTAC
421      GCCTACGACG GCAAGGATTA CATCGCCCTG AAAGAGGACC TGCGCCCTTG GACCGCGGCG
481      GACATGGCAG CTCAGACCAC CAAGCACAAG TGGGAGGCGG CCCATGTGGC GGAGCAGTTG
541      AGAGCCTACC TGGAGGGCAC GTGCGTGGAG TGGCTCCGCA GATACCTGGA GAACGGGAAG
601      GAGACGCTGC AGCGCACGGA CGCCCCCAAA ACGCATATGA CTCACCACGC TGTCTCTGAC
661      CATGAAGCCA CCCTGAGGTG CTGGGCCCTG AGCTTCTACC CTGCGGAGAT CACACTGACC
721      TGGCAGCGGG ATGGGGAGGA CCAGACCCAG GACACGGAGC TCGTGGAGAC CAGGCCTGCA
781      GGGGATGGAA CCTTCCAGAA GTGGGCGGCT GTGGTGGTGC CTTCTGGACA GGAGCAGAGA
841      TACACCTGCC ATGTGCAGCA TGAGGGTTTG CCCAAGCCCC TCACCCTGAG ATGGGAGCCG
901      TCTTCCCAGC CCACCATCCC CATCGTGGGC ATCATTGCTG GCCTGGTTCT CTTTGGAGCT
961      GTGATCACTG GAGCTGTGGT CGCTGCTGTG ATGTGGAGGA GGAAGAGCTC AGATAGAAAA
1021     GGAGGGAGCT ACTCTCAGGC TGCAAGCAGT GACAGTGCCC AGGGCTCTGA TGTGTCTCTC
1081     ACAGCTTGTA AAGTGTGA

Quality Control:cDNA sequencing results is available upon request.