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Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set
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Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set PDF Download

Catalog   Size (Price) Quantity In Stock Operation
SEK11233  

YES   
 

HIV-1 gp120 / Glycoprotein 120 ELISA Pair Set

Order or Inquire for HIV-1 gp120 / Glycoprotein 120 ELISA Pair Set
High affinity HIV-1 gp120 / Glycoprotein 120 ELISA Pair Set
Detection limit - 39 pg/ml
Affordable price and 30%-80% cost saving for Bulk order
  • Materials
  • Protocol
  • Trouble Shooting
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Problems

Possible Sources

Solutions

No signal

Incorrect or no Detection Antibody was added

Add appropriate Detection Antibody and continue

Substrate solution was not added

Add substrate solution and continue

Incorrect storage condition

Check if the kit is stored at recommended condition and used before expiration date

     

Poor Standard Curve

Standard was incompletely reconstituted or was inappropriately stored

Aliquot reconstituted standard and store at  -70 ℃

Imprecise / inaccurate pipetting

Check / calibrate pipettes

Incubations done at inappropriate temperature, timing or agitation

Follow the general ELISA protocol

Background wells were contaminated

Avoid cross contamination by using the sealer appropriately

     

Poor detection value

The concentration of antigen in samples was too low

Enriching samples to increase the concentration of antigen

Samples were ineffective

Check if the samples are stored at cold environment. Detect samples in timely manner

     

High Background

Insufficient washes

Use multichannel pipettes without touching the reagents on the plate

Increase cycles of washes and soaking time between washes

TMB Substrate Solution was contaminated

TMB Substrate Solution should be clear and colorless prior to addition to wells

Materials were contaminated

Use clean plates, tubes and pipettes tips

     

Non-specificity

Samples were contaminated

Avoid cross contamination of samples

The concentration of samples was too high

Try higher dilution rate of samples

 

 

HIV-1 gp120 / Glycoprotein 120 ELISA Pair Set Related Products & Topics

Related Areas:

Proteins:

Antibodies:

Background

Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses.
The HIV-1 surface protein gp120, also known as Glycoprotein 120, SU, and gp120 is not anchored to the viral envelope, but associates with the extravirion surface through its binding to TM. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. Surface protein gp120 is a ligand for CD209 / DC-SIGN and CLEC4M / DC-SIGNR. It may target the virus to gut-associated lymphoid tissue (GALT) by binding host ITGA4/ITGB7 (alpha-4/beta-7 integrins), a complex that mediates T-cell migration to the GALT. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"