HIV-1 Gag-p24 Antibody

Gag-p24 Antibody Datasheet

Gag-p24 Antibody Product Information

• Immunogen: Recombinant HIV-1 Gag-P24 protein (Catalog#11695-V08E)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 9B6C2B7
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HIV-1 Gag-p24 (HIV-1 Gag-p24; Catalog#11695-V08E; ACI05538.1; Pro 133-Leu 363). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Gag-p24 Antibody Usage Guide

• Specificity: HIV-1 Gag-p24
• No cross-reactivity in ELISA with

  E.coli cell lysate

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect HIV-1 Gag-p24 in WB. Using a DAB detection system, the detection limit for HIV-1 Gag-p24 is approximately 20 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HIV-1 Gag-p24. The detection limit for HIV-1 Gag-p24 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gag-p24 Antibody Background

Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses. Gag protein from HIV-1 is a polyprotein, which, during viral maturation, is cleaved to release matrix p17, core p24 and nucleocapsid proteins. Capsid protein p24 forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is dissassembled soon after virion entry. The p24 antigen contains epitopes that prime helper CD4 T-cells, which have been demonstrated to be protective and it can elicit lymphocyte proliferation. p24 is likely to be an integral part of any multicomponent HIV vaccine.

References

1. Yang X., J. et al., 2005, Virol. 79: 12132-47.
2. Beddows, S. et al., 2007,Virology. 360 (2): 329-40.
3. Mendu, DR. et al., 2007,Biochem Biophys Res Commun. 363 (3): 466-71.
4. Du, SX. et al., 2009, Virology. 395 (1): 33-44.
5. Donayre-Torres, A.J. et al., 2009, Virol J. 6:17.