HIV-1 GP120 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
VG11233-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HIV-1 GP120 cDNA Clone Datasheet

HIV-1 GP120 cDNA Clone Product Information

cDNA Size:	1540
Gene Synonym:	gp120
Source:	Human Immunodeficiency Virus type 1 (HIV-1)

HIV-1 GP120 cDNA Clone Description

cDNA Description:	ORF Clone of HIV-1 envelope glycoprotein DNA.
Vector:	Cloning Vector: pGEM-T Vector. Restriction site: KpnI + XbaI Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Similar to CN97001

HIV-1 GP120 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HIV-1 GP120 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

HIV-1 GP120 cDNA Clone Sequence and Quality Control

Sequence:

1        CGAGGTACCA TGAGAGTGAC GGGGATCAGG AAGAATTATC GGCATTTATG GAGATGGGGC
61       ACCATGCTCC TTGGGATGTT GATGATCAGT AGTGCCGTGG GCAACCTGTG GGTGACCGTG
121      TACTACGGCG TGCCCGTGTG GAAGGGCGCC ACCACCACCG AGTTCTGCGC CAGCGACGCC
181      AAGGCCTACG ACACCGAGGT GCACAACGTG TGGGCCACCC ACGCCTGCGT GCCCGCCGAC
241      CCCAACCCCC AGGAGATGGT GCTGGAGAAC GTGACCGAGA ACTTCAACAT GTGGAAGAAC
301      GAGATGGTGA ACCAGATGCA GGAGGACGTC ATCAGCCTGT GGGACCAGAG CCTGAAGCCC
361      TGCGTGAAGC TGACCCCCCT GTGCGTGACC CTGGAGTGCA GGAACGTGAG CAGCAACCAG
421      GACACCTACC ACGAGACCTA CCACGAGAGC ATGAAGGAGA TGAAGAACTG CAGCTTCAAC
481      GCCACCACCG TGGTGGAGGA CAGGAAGCAG ACCGTGTACG CCCTGTTCTA CAGGCTGGAC
541      ATCGTGCCCC TGACCAAGAA GAACTACGAG AAGAACAGCC AGGAGTACTA CAGGCTGATC
601      AACTGCAACA CCAGCGCCAT CACCCAGGCC TGCCCCAAGG TGACCTTCGA CCCCATCCCC
661      ATCCACTACT GCACCCCCGC CGGCTACGCC ATCCTGAAGT GCAACGACAA GATCTTCAAC
721      CGCACCCAGC CCTGCTACAA CGTGAGCACC GTGCAGTGCA CCCACGGCAT CAAGCCCGTG
781      GTGAGCACCC AGCTGCTGCT GAACGGCAGC CTGGCCGAGG GCGAGATCAT CATCAGGAGC
841      GAGAACCTGA CCAACAACGT GAAAACCATC ATCGTGCACC TGAACCAGAG CGTGGAGATC
901      GTGTGCACCA GGCCCGGCAA CAACACCAGG AAGAGCATCA GGATCGGCCC CGGCCAGACC
961      TTCTACGCCA CCGGCGACAT CATCGGCGAC ATCAGGCAGG CCCACTGCAA CATCAGCGAG
1021     GACAAGTGGA ACGAGACCCT GCAGAGGGTG AGCAAGAAGC TTGCCGAGCA CTTCCAGAAC
1081     AAGACCATCA AGTTCGCCAG CAGCAGCGGC GGCGACCTGG AGGTGACCAC CCACAGCTTC
1141     AACTGCAGGG GCGAGTTCTT CTACTGCAAC ACCAGCGGCC TGTTCAACGG CGCCTACACC
1201     CCCAACGGCA CCAAGAGCAA CAGCAGCAGC ATCATCACCA TCCCCTGCAG GATCAAGCAG
1261     ATCATCAACA TGTGGCAGGA GGTGGGCAGG GCCATGTACG CCCCTCCCAT CAAGGGCAAC
1321     ATCACCTGCA AGAGCAACAT CACCGGCCTG CTGCTGGTGA GGGACGGCGG CACCGAGCCC
1381     AACGACACCG AGACCTTCAG GCCCGGCGGC GGCGACATGA GGAACAACTG GAGGAGCGAG
1441     CTGTACAAGT ACAAGGTGGT GGAGATCAAG CCCCTGGGCG TGGCCCCCAC CACCACCAAG
1501     AGGAGGGTGG TGGAGAGAGA AAAGCGCTAA ACTCGAGCTG

Quality Control:cDNA sequencing results is available upon request.