Mouse HIST3H2A Gene cDNA Clone / ORF Clone

HIST3H2A cDNA Clone Datasheet

HIST3H2A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_178218.3
• cDNA Size: 393
• Gene Synonym: Hist3h2a
• Source: Mouse

HIST3H2A cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus histone cluster 3, H2a DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 132 C/G and 288 G/A, not causing the amino acid variation.

HIST3H2A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HIST3H2A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HIST3H2A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTGGTC GTGGCAAGCA GGGCGGCAAA GCTCGTGCAA AAGCGAAGTC TCGTTCTTCC
61       CGTGCCGGCC TGCAGTTTCC AGTGGGCCGT GTGCACCGCC TTCTCCGTAA GGGTAATTAT
121      TCGGAGCGGG TGGGCGCTGG TGCCCCGGTG TACCTGGCGG CTGTGCTAGA ATATCTGACT
181      GCTGAGATCC TGGAATTGGC TGGTAATGCG GCCCGCGACA ATAAGAAGAC GCGAATCATC
241      CCGCGCCACC TGCAGTTGGC CATCCGCAAC GACGAGGAGC TCAACAAACT GCTGGGCCGC
301      GTGACCATCG CGCAGGGCGG CGTCCTGCCC AACATCCAGG CCGTGCTACT GCCCAAGAAG
361      ACCGAGAGCC ACCACAAGGC CAAGGGCAAG TGA

 

Quality Control:cDNA sequencing results is available upon request.