HIST3H2A / Histone H2A Protein (Native)

Histone cluster 3, H2a Protein Datasheet

HIST3H2A / Histone H2A Protein Product Information

• Synonym: MGC3165
• Protein Construction: A DNA sequence encoding the human HIST3H2A (NP_254280.1) (Met 1-Lys 130) was expressed and purified.
• Source: Human
• Expression Host: E. Coli

HIST3H2A / Histone H2A Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human HIST3H2A consists of 130 amino acids and has a calculated molecular mass of 14.2 KDa as estimated in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM β-Mercaptoethanol, pH 6.9
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: HIST3H2A / Histone H2A protein

HIST3H2A / Histone H2A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

HIST3H2A / Histone H2A Protein Description

Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. Histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone cluster 3, H2a also known as histone H2A (HIST3H2A) is a member of histones. Covalent modification of histones is important in regulating chromatin dynamics and transcription. One example of such modification is ubiquitination, which mainly occurs on histones H2A and H2B. E3 ubiquitin ligase complex is specific for histone H2A (HIST3H2A). Reducing the expression of Ring2 results in a dramatic decrease in the level of ubiquitinated H2A in HeLa cells.DNA damage induces monoubiquitylation of histone H2A (HIST3H2A) in the vicinity of DNA lesions.

References

1. Fuchs TA. et al., 2011, Blood. 118 (13): 3708-14.
2. Collart D. et al., 1993, J Cell Biochem. 50 (4): 374–85.
3. Marzluff WF. et al., 2002, Genomics. 80 (5): 487–98.
4. Wang HB. et al., 2004, Nature. 431: 873-8.