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Histone H3 Antibody, Mouse MAb

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Human Histone H3 Antibody Product Information
Immunogen:A synthetic peptide corresponding to the C-terminus of the human Histone H3.
Clone ID:1F6D7C4
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human Histone H3 Antibody Usage Guide
Specificity:Human Histone H3
Application:WB, IHC-P, IP

WB: 1/1000-1/10000

IHC-P: 1/50-1/1000

IP: 4-10 uL/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human Histone H3 Antibody WB Application Image
Human Histone H3 Antibody IHC Application Image
Histone H3 Antibody, Mouse Mab, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Histone H3 Antibody, Mouse Mab, Immunochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).

Human Histone H3 Antibody IP Application Image
Other Histone H3 Antibody Products
Histone H3 Background

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.

Human Histone H3 References
  • Bhasin M, et al., 2006, 13(1): 102-12.
  • Lachner M, et al., 2001, 410(6824): 116-20.
  • Rosenfeld JA, et al., 2009, BMC Genomics. 10: 143.
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    Catalog: 100005-MM01-50
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"