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HIST1H3A Antibody, Rabbit PAb, Antigen Affinity Purified
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HIST1H3A Antibody, Rabbit PAb, Antigen Affinity Purified PDF Download

Catalog   Size (Price) Quantity In Stock Operation
11231-RP02  

YES   
 

Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

HIST1H3A Antibody ( Antigen Affinity Purified ) Datasheet

  Order or Inquire for HIST1H3A Antibody product Quality antibodies Antibody production services
  Detection limit is 0.0039 ng/well in ELISA
 

HIST1H3A Antibody Product Information

Immunogen :

Recombinant Human HIST1H3A protein (Catalog#11231-HNAE)

Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type :

Rabbit IgG

Formulation : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
Preparation :

Produced in rabbits immunized with purified, recombinant Human HIST1H3A (rh HIST1H3A; Catalog#11231-HNAE; P6843; Met 1-Ala 136). HIST1H3A specific IgG was purified by Human HIST1H3A affinity chromatography.

HIST1H3A Antibody Usage Guide

Specificity :

Human HIST1H3A

Immunochemistry :

IHC-P: 0.1-2 μg/mL

 

H3A-bladder-carcinoma immunohistochemistry    H3A-colon-carcinoma immunohistochemistry

Immunochemical staining of human H3A in human bladder carcinoma with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. Immunochemical staining of human H3A in human colon carcinoma with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.

 

H3A-colon immunohistochemistry    

Immunochemical staining of human H3A in human colon with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.  
Direct ELISA : This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human H3A/HIST1H3A. The detection limit for Human H3A/HIST1H3A is approximately 0.0039 ng/well.
Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HIST1H3A Antibody Related Products & Topics

Related Areas:

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
HIST1H3A Human HIST1H3A /Histone H3.1 Protein, Recombinant 11231-HNAE

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human HIST1H3A ELISA, IHC-P HIST1H3A Antibody (Antigen Affinity Purified) 11231-RP02

HIST1H3A Antibody Background

Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of thehistone H3 family which is a core component of nucleosome. It is expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

References

  1. 1. Lachner M, et al., 2001, Nature410 (6824): 116-20.
  2. Koessler H, et al., 2003, DNA Cell Biol.22 (4): 233-41.
  3. Macdonald N. et al., 2005, Mol. Cell 20: 199-211.
  4. Hyllus D. et al., 2007, Genes Dev. 21: 3369-3380.
  5. Garcia BA. et al., 2007, J. Biol. Chem. 282:7641-7655.
  6. Yu L.-R. et al.,2007, J. Proteome Res. 6: 4150-4162.
 

 

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