Anti-HIST1H3A Antibody

Cat: 11231-RP02

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Anti-HIST1H3A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-HIST1H3A Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human HIST1H3A
Immunogen
Recombinant Human HIST1H3A protein (Catalog#11231-HNAE)
Preparation
Produced in rabbits immunized with purified, recombinant Human HIST1H3A (rh HIST1H3A; Catalog#11231-HNAE; NP_003520.1; Met 1-Ala 136). HIST1H3A specific IgG was purified by Human HIST1H3A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HIST1H3A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 2-10 μg/mL  
ELISA 0.1-0.2 μg/mL  
IHC-P 0.1-2 μg/mL  
IP 1-4 μg/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HIST1H3A Antibody (Rabbit Polyclonal antibody) Images
Human HIST1H3A Immunohistochemistry(IHC) 4078
Immunochemical staining of human H3A in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Human HIST1H3A Immunohistochemistry(IHC) 4079
Immunochemical staining of human H3A in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Human HIST1H3A Immunohistochemistry(IHC) 4080
Immunochemical staining of human H3A in human bladder carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.
Human HIST1H3A Immunoprecipitation(IP) 8072
Human HIST1H3A Western blot (WB) 13374
Anti-HIST1H3A Antibody Alternative Names
Anti-H3/A Antibody;Anti-H3FA Antibody;Anti-HIST1H3A Antibody;Anti-HIST1H3B Antibody;Anti-HIST1H3C Antibody;Anti-HIST1H3D Antibody;Anti-HIST1H3E Antibody;Anti-HIST1H3F Antibody;Anti-HIST1H3G Antibody;Anti-HIST1H3H Antibody;Anti-HIST1H3I Antibody;Anti-HIST1H3J Antibody
HIST1H3A Background Information

Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Full Name
histone cluster 1, H3a
References
  • Lachner M, et al., 2001, Nature 410 (6824): 116-20.
  • Koessler H, et al., 2003, DNA Cell Biol. 22 (4): 233-41.
  • Macdonald N. et al., 2005, Mol. Cell 20: 199-211.
  • Hyllus D. et al., 2007, Genes Dev. 21: 3369-3380.
  • Garcia BA. et al., 2007, J. Biol. Chem. 282:7641-7655.
  • Yu L.-R. et al., 2007, J. Proteome Res. 6: 4150-4162.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-HIST1H3A Antibody Rabbit Polyclonal WB,ELISA,IHC-P 11231-T56
    Anti-HIST1H3A Antibody Rabbit Polyclonal ELISA 11231-RP01
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