Anti-HIF-1 alpha Antibody

Cat: 11977-RP02
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All HIF-1 alpha Reagents
CRO Services
Product recommended

All HIF-1 alpha Reagents
Anti-HIF-1 alpha Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-HIF-1 alpha Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human HIF-1 alpha
Immunogen
Recombinant Human HIF1A protein (Catalog#11977-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human HIF1A (rh HIF1A; Catalog#11977-H07E; Q16665-1; Arg 575-Asn 826). HIF1A specific IgG was purified by human HIF1A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HIF-1 alpha Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HIF-1 alpha Antibody Alternative Names
Anti-bHLHe78 Antibody;Anti-HIF-1A Antibody;Anti-HIF-1alpha Antibody;Anti-HIF1 Antibody;Anti-HIF1-ALPHA Antibody;Anti-MOP1 Antibody;Anti-PASD8 Antibody
HIF-1 alpha Background Information

HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain and 2 PAS (PER-ARNT-SIM) domains. It is one of the two subunits of Hypoxia-inducible factor-1 (HIF1). HIF1 is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF-1 alpha is expressed in most tissues with highest levels in kidney and heart. It is overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, it activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. HIF-1 alpha binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300.

Full Name
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
References
  • Zhou Q, et al. (2011) Loss of either hypoxia inducible factor 1 or 2 promotes lung cancer cell colonization. Cell Cycle. 10(13):2233-4.
  • Krishnan J, et al. (2012) Dietary obesity-associated Hif1 alpha activation in adipocytes restricts fatty acid oxidation and energy expenditure via suppression of the Sirt2-NAD+ system. Genes Dev. 26(3):259-70.
  • Novo E, et al. (2012) The biphasic nature of hypoxia-induced directional migration of activated human hepatic stellate cells. J Pathol. 226(4):588-97.
  • Dungwa JV, et al. (2011) Overexpression of carbonic anhydrase and HIF-1 in Wilms tumours. BMC Cancer. 11:390.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-HIF-1 alpha Antibody Rabbit Monoclonal ICC/IF,IF 11977-R004
    Anti-HIF-1 alpha Antibody Rabbit Polyclonal ELISA 11977-RP01
    Anti-HIF-1 alpha Antibody Mouse Monoclonal IHC-P 11977-MM01
    info info
    添加购物车成功! 添加购物车失败!