Human HHIP Gene cDNA Clone / ORF Clone

HHIP cDNA Clone Datasheet

HHIP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_022475.2
• cDNA Size: 2103
• Gene Synonym: HIP; HHIP
• Source: Human

HHIP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens hedgehog interacting protein DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

HHIP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HHIP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HHIP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA
61       GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG
121      AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG
181      CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG
241      CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC
301      ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA
361      AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC
421      TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT
481      CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT
541      CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA
601      GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG
661      GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA
721      CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT
781      TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT
841      GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT
901      GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG
961      GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA
1021     GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC
1081     CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA
1141     GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC
1201     ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG
1261     CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT
1321     CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA
1381     TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT
1441     TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC
1501     CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT
1561     CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT
1621     GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT
1681     GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC
1741     AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT
1801     GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG
1861     GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG
1921     CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA
1981     TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGGT
2041     ATTCTTGATC AGATCATTGA CATGACATCT TACTTGCTGG ATCTAACAAG TTACATTGTA
2101     TAG

 

Quality Control:cDNA sequencing results is available upon request.