pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human HGF ORF mammalian expression plasmid, C-GFPSpark tag||HG10463-ACG|
|Human HGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10463-ACR|
|Human HGF ORF mammalian expression plasmid, C-Flag tag||HG10463-CF|
|Human HGF ORF mammalian expression plasmid, C-His tag||HG10463-CH|
|Human HGF ORF mammalian expression plasmid, C-Myc tag||HG10463-CM|
|Human HGF ORF mammalian expression plasmid, C-HA tag||HG10463-CY|
|Human HGF ORF mammalian expression plasmid, N-Flag tag||HG10463-NF|
|Human HGF ORF mammalian expression plasmid, N-His tag||HG10463-NH|
|Human HGF ORF mammalian expression plasmid, N-Myc tag||HG10463-NM|
|Human HGF ORF mammalian expression plasmid, N-HA tag||HG10463-NY|
|Human HGF natural ORF mammalian expression plasmid||HG10463-UT|
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Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.