HGF Protein, Rat, Recombinant

Cat: 80429-RNAH
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HGF Protein, Rat, Recombinant Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Measured in a cell proliferation assay using Mv-1-1u mink lung epithelial cells.
The ED50 for this effect is typically 1-6 ng/mL.
Protein Construction
A DNA sequence encoding the rat HGF (Met1-Leu728) was expressed and purified.
Expressed Host
HEK293 Cells
Species
Rat
Predicted N Terminal
Gln 33
Molecule Mass
The recombinant rat HGF consists of 698 amino acids and has a predicted molecular mass of 79.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 92 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
HGF Protein, Rat, Recombinant in SDS-PAGE
Rat HGF/Hepatocyte Growth Factor Protein 12631
HGF Background Information

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
hepatocyte growth factor (hepapoietin A; scatter factor)
References
  • Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
  • Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
  • Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
  • Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
  • Other HGF Proteins

    Canine HGF Proteins

    Product Name Cat.No.
    HGF Protein, Canine, Recombinant 70063-DNAH

    Cynomolgus HGF Proteins

    Product Name Cat.No.
    HGF Protein, Cynomolgus, Recombinant 90286-CNAH

    Mouse HGF Proteins

    Product Name Cat.No.
    HGF Protein, Mouse, Recombinant (His Tag) 50038-M08H
    HGF Protein, Mouse, Recombinant 50038-MNAH

    Human HGF Proteins

    Product Name Cat.No.
    HGF Protein, Human, Recombinant 10463-HNAS
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