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HGF / Hepatocyte Growth Factor Antibody Datasheet
|Order or Inquire for HGF / Hepatocyte Growth Factor Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.078 ng/well in ELISA|
HGF / Hepatocyte Growth Factor Antibody Product Information
Recombinant Human HGF / Hepatocyte Growth Factor protein (Catalog#10463-H08H)
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HGF / Hepatocyte Growth Factor (rh HGF / Hepatocyte Growth Factor; Catalog#10463-H08H; NP_000592.3; Met 1-Ser 728). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
HGF / Hepatocyte Growth Factor Antibody Usage Guide
Human HGF / Hepatocyte Growth Factor
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
|Direct ELISA :||This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human HGF. The detection limit for Human HGF is approximately 0.078 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
HGF / Hepatocyte Growth Factor Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|HGF||Human||HGF Protein, Recombinant||10463-H08H|
|HGF||Human||HGF Protein, Recombinant||10463-HNAS|
|HGF||Mouse||HGF / Hepatocyte Growth Factor Protein, Recombinant||50038-M08H|
|HGF||Rat||HGF / Hepatocyte Growth Factor Protein, Recombinant, with Native||80429-RNAH|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human HGF||WB, ELISA||HGF Antibody||10463-RP01|
|Human HGF||WB, ELISA||HGF / Hepatocyte Growth Factor Antibody (Biotin conjugated)||10463-RP01-B|
|Human HGF||WB, ELISA||HGF Antibody (Antigen Affinity Purified)||10463-RP02|
|Human HGF||WB, ELISA||HGF Antibody||10463-RP03|
|Human HGF||WB, ELISA||HGF Antibody (Antigen Affinity Purified)||10463-RP04|
|Human HGF||WB, ELISA||HGF / Hepatocyte Growth Factor Antibody||10463-R007|
|Human HGF||ELISA||HGF / Hepatocyte Growth Factor Antibody||10463-MM04|
HGF / Hepatocyte Growth Factor Antibody Background
Hepatocyte growth factor (HGF), also known as Scatter factor, is a paracrine cellular growth, motility and morphogenic factor. Hepatocyte Growth Factor/HGF is a member of the peptidase S1 family, and is derived from a single chain precursor of 728 amino acid residues containing 4 kringle domains, 1PAN domain and 1peptidase S1 domain. The synthesized single chain of HGF is further processed into a bioactive heterodimer composed of the 69 kDa α-subunit and the 34 kDa β-subunit by a serine protease. Hepatocyte Growth Factor/HGF has no detectable protease activity. Alternative splicing of its gene produces multiple transcript variants encoding different isoforms. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, as well as haemopoietic progenitor cells. Hepatocyte Growth Factor/HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. Hepatocyte Growth Factor/HGF may also play an important role as a paracrine or endocrine mediator through an epithelial-mesenchymal interaction in wound-healing, tissue or organ regeneration, morphogenesis and carcinogenesis.
- Naldini, L. et al.,1991, EMBO J. (ENGLAND)10 (10): 2867-78.
- Nakamura, T. et al., 1992,Prog. Growth Factor Res.3 (1): 67-85.
- Comoglio, PM. et al., 1993, EXS. (SWITZERLAND)65: 131-65.
- Skibinski, G. et al., 2004, Arch. Immunol. Ther. Exp. 51 (5): 277-82.
- Kemp, LE. et al., 2006, Biochem. Soc. Trans.34 (Pt 3): 414-7.