HGF / Hepatocyte Growth Factor Antibody

HGF / Hepatocyte Growth Factor Antibody Datasheet

HGF / Hepatocyte Growth Factor Antibody Product Information

• Immunogen: Recombinant Human HGF / Hepatocyte Growth Factor protein (Catalog#10463-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 04
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HGF / Hepatocyte Growth Factor (rh HGF / Hepatocyte Growth Factor; Catalog#10463-H08H; NP_000592.3; Met 1-Ser 728). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

HGF / Hepatocyte Growth Factor Antibody Usage Guide

• Specificity: Human HGF / Hepatocyte Growth Factor
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human HGF. The detection limit for Human HGF is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HGF / Hepatocyte Growth Factor Antibody Background

Hepatocyte growth factor (HGF), also known as Scatter factor, is a paracrine cellular growth, motility and morphogenic factor. Hepatocyte Growth Factor/HGF is a member of the peptidase S1 family, and is derived from a single chain precursor of 728 amino acid residues containing 4 kringle domains, 1PAN domain and 1peptidase S1 domain. The synthesized single chain of HGF is further processed into a bioactive heterodimer composed of the 69 kDa α-subunit and the 34 kDa β-subunit by a serine protease. Hepatocyte Growth Factor/HGF has no detectable protease activity. Alternative splicing of its gene produces multiple transcript variants encoding different isoforms. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, as well as haemopoietic progenitor cells. Hepatocyte Growth Factor/HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. Hepatocyte Growth Factor/HGF may also play an important role as a paracrine or endocrine mediator through an epithelial-mesenchymal interaction in wound-healing, tissue or organ regeneration, morphogenesis and carcinogenesis.

References

1. Naldini, L. et al.,1991, EMBO J. (ENGLAND)10 (10): 2867-78.
2. Nakamura, T. et al., 1992,Prog. Growth Factor Res.3 (1): 67-85.
3. Comoglio, PM. et al., 1993, EXS. (SWITZERLAND)65: 131-65.
4. Skibinski, G. et al., 2004, Arch. Immunol. Ther. Exp. 51 (5): 277-82.
5. Kemp, LE. et al., 2006, Biochem. Soc. Trans.34 (Pt 3): 414-7.