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Your Position: Home > Gene Products > Other Species > Cynomolgus monkey HFE2 Gene cDNA Clone / ORF Clone

Cynomolgus monkey HFE2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
CG90061-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HFE2 cDNA Clone Datasheet

HFE2 cDNA Clone Product Information

cDNA Size:	1284
Gene Synonym:	HFE2
Source:	Cynomolgus monkey

HFE2 cDNA Clone Description

cDNA Description:	ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) hemochromatosis type 2 (juvenile) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1007 C/G resulting in the amino acid Pro substitution by Arg. Please check the sequence information before order.

HFE2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HFE2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HFE2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGAGC AAGGCCAGTC CCCTAGTCCC AGGTCCTCCC ATGGCAGTCC CCCAACTCTA
61       AGCACTCTCA CTCTCCTGCT GCTCCTCTGT GGACATGCTC ATTCTCAATG CAAGATCCTC
121      CGCTGCAATG CTGAGTATGT ATCGTCCACT CTGAGCCTTA GAGGTGGCGG TTCATCAGGA
181      GCACTTCGAG GAGGAGGAGG AGGAGGAGGC CGGGGTGGAG GGGTGGGCTC TGGCGGCCTC
241      TGTCGAGCCC TCCGCTCCTA TGCGCTCTGC ACTCGGCGCA CCGCCCGCAC CTGCCGTGGG
301      GACCTCGCCT TCCATTCGGC GGTACATGGC ATCGAAGACC TGATGATCCA GCACAACTGC
361      TCGCGCCAGG GCCCTACAGC CCCTCCCCCG CCCCGGGGCC CCGCCCTTCC AGGCGCAGGC
421      TCCGGCCTCC CTGCCCCGGA CCCTTGTGAC TATGAAGGCC GGTTTTCCCG GCTGCATGGT
481      CGTCCCCCGG GGTTCTTGCA TTGCGCTTCC TTCGGGGACC CCCATGTGCG CAGCTTCCAC
541      CACCATTTTC ACACATGCCG TGTCCAAGGA GCTTGGCCTC TACTGGATAA CGACTTCCTC
601      TTTGTCCAAG CCACCAGCTC CCCCATGGCG TTGGGGGCCA ACGCTACCGC TACCCGGAAG
661      CTCACCATCA TATTTAAGAA CATGCAGGAA TGCATTGATC AGAAGGTCTA TCAGGCTGAG
721      GTGGATAATC TTCCTGCAGC CTTTGAAGAT GGTTCTGTCA ATGGAGGTGA CCGACCTGGG
781      GGATCCAGTT TGTCGATTCA AACTGCTAAC CCTGGGAACC ACGTGGAGAT CCAAGCTGCC
841      TACATTGGCA CAACTATAAT CATTCGGCAG ACAGCTGGGC AGCTCTCCTT CTCCATCAAG
901      GTAGCAGAGG ATGTGGCCAT GGCCTTCTCA GCTGAACAGG ACCTGCAGCT CTGTGTTGGG
961      GGGTGCCCTC CAAGTCAGCG ACTCTCTCGA TCAGAGCGCA GTCGTCGGGG AGCTATAACC
1021     ATTGATACTG CCAGACGGCT GTGTAAGGAA GGGCTTCCAG TGGAAGATGC TTACTTCCAT
1081     TCCTGTGTCT TTGATGTTTT AATTTCTGGT GATCCCAACT TTACTGTGGC AGCTCAGGCA
1141     GCACTGGAGG ATGCCCGAGC CTTCCTGCCA GACTTAGATA AGCTGCATCT CTTCCCTTCA
1201     GATGCTGGGG TTTCTCTTTC CTCAGCAACC TTCCTAGCCC CACTCCTTTC TGGGCTCTTT
1261     GTTCTGTGGC TTTGCATTCA GTAA

Quality Control:cDNA sequencing results is available upon request.