Human HEXB Gene cDNA Clone / ORF Clone

HEXB cDNA Clone Datasheet

HEXB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_000521.3
• cDNA Size: 1671
• Gene Synonym: ENC-1AS
• Source: Human

HEXB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens hexosaminidase B (beta polypeptide) DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

HEXB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HEXB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HEXB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTGT GCGGGCTGGG GCTGCCCCGG CCGCCCATGC TGCTGGCGCT GCTGTTGGCG
61       ACACTGCTGG CGGCGATGTT GGCGCTGCTG ACTCAGGTGG CGCTGGTGGT GCAGGTGGCG
121      GAGGCGGCTC GGGCCCCGAG CGTCTCGGCC AAGCCGGGGC CGGCGCTGTG GCCCCTGCCG
181      CTCTCGGTGA AGATGACCCC GAACCTGCTG CATCTCGCCC CGGAGAACTT CTACATCAGC
241      CACAGCCCCA ATTCCACGGC GGGCCCCTCC TGCACCCTGC TGGAGGAAGC GTTTCGACGA
301      TATCATGGCT ATATTTTTGG TTTCTACAAG TGGCATCATG AACCTGCTGA ATTCCAGGCT
361      AAAACCCAGG TTCAGCAACT TCTTGTCTCA ATCACCCTTC AGTCAGAGTG TGATGCTTTC
421      CCCAACATAT CTTCAGATGA GTCTTATACT TTACTTGTGA AAGAACCAGT GGCTGTCCTT
481      AAGGCCAACA GAGTTTGGGG AGCATTACGA GGTTTAGAGA CCTTTAGCCA GTTAGTTTAT
541      CAAGATTCTT ATGGAACTTT CACCATCAAT GAATCCACCA TTATTGATTC TCCAAGGTTT
601      TCTCACAGAG GAATTTTGAT TGATACATCC AGACATTATC TGCCAGTTAA GATTATTCTT
661      AAAACTCTGG ATGCCATGGC TTTTAATAAG TTTAATGTTC TTCACTGGCA CATAGTTGAT
721      GACCAGTCTT TCCCATATCA GAGCATCACT TTTCCTGAGT TAAGCAATAA AGGAAGCTAT
781      TCTTTGTCTC ATGTTTATAC ACCAAATGAT GTCCGTATGG TGATTGAATA TGCCAGATTA
841      CGAGGAATTC GAGTCCTGCC AGAATTTGAT ACCCCTGGGC ATACACTATC TTGGGGAAAA
901      GGTCAGAAAG ACCTCCTGAC TCCATGTTAC AGTAGACAAA ACAAGTTGGA CTCTTTTGGA
961      CCTATAAACC CTACTCTGAA TACAACATAC AGCTTCCTTA CTACATTTTT CAAAGAAATT
1021     AGTGAGGTGT TTCCAGATCA ATTCATTCAT TTGGGAGGAG ATGAAGTGGA ATTTAAATGT
1081     TGGGAATCAA ATCCAAAAAT TCAAGATTTC ATGAGGCAAA AAGGCTTTGG CACAGATTTT
1141     AAGAAACTAG AATCTTTCTA CATTCAAAAG GTTTTGGATA TTATTGCAAC CATAAACAAG
1201     GGATCCATTG TCTGGCAGGA GGTTTTTGAT GATAAAGCAA AGCTTGCGCC GGGCACAATA
1261     GTTGAAGTAT GGAAAGACAG CGCATATCCT GAGGAACTCA GTAGAGTCAC AGCATCTGGC
1321     TTCCCTGTAA TCCTTTCTGC TCCTTGGTAC TTAGATTTGA TTAGCTATGG ACAAGATTGG
1381     AGGAAATACT ATAAAGTGGA ACCTCTTGAT TTTGGCGGTA CTCAGAAACA GAAACAACTT
1441     TTCATTGGTG GAGAAGCTTG TCTATGGGGA GAATATGTGG ATGCAACTAA CCTCACTCCA
1501     AGATTATGGC CTCGGGCAAG TGCTGTTGGT GAGAGACTCT GGAGTTCCAA AGATGTCAGA
1561     GATATGGATG ACGCCTATGA CAGACTGACA AGGCACCGCT GCAGGATGGT CGAACGTGGA
1621     ATAGCTGCAC AACCTCTTTA TGCTGGATAT TGTAACCATG AGAACATGTA A

 

Quality Control:cDNA sequencing results is available upon request.