HER3 / ErbB3 Antibody

HER3 / ErbB3 Antibody Datasheet

HER3 / ErbB3 Antibody Product Information

• Immunogen: Recombinant Human HER3 / ErbB3 Protein （Catalog #10201-H08H）
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 016
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human HER3 / ErbB3 (rh HER3 / ErbB3; Catalog#10201-H08H; Met 1 - Thr 643; NP_001973.2)

HER3 / ErbB3 Antibody Usage Guide

• Specificity: Human HER3 / ErbB3
• No cross-reactivity in ELISA with

  Human EGFR / ErbB1 / HER1

  Human ErbB4 / HER4

  Human ErbB2 / HER2

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ErbB3 in WB. Using a DAB detection system, the detection limit for Human ErbB3 is approximately 1 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ErbB3.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HER3 / ErbB3 Antibody Background

ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

References

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4. Alimandi, M. et al., 1995, Oncogene. 10:1813-1821.
5. Gamett, D.C. et al., 1995, J. Biol. Chem. 270 : 19022-19027.
6. Katoh, M. et al., 1993, Biochem.Biophys.Res.Commun.192: 1189-97.