HER2 / ErbB2 Antibody (Antigen Affinity Purified)

HER2 / ErbB2 Antibody ( Antigen Affinity Purified ) Datasheet

HER2 / ErbB2 Antibody Product Information

• Immunogen: Recombinant Rhesus HER2 / ErbB2 protein (Catalog#90020-K08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Rhesus HER2 / ErbB2 (rRhesus HER2 / ErbB2; Catalog#90020-K08H; XP_001090430.1; Met 1-Thr 652). HER2 / ErbB2 specific IgG was purified by Rhesus HER2 / ErbB2 affinity chromatography.

HER2 / ErbB2 Antibody Usage Guide

• Specificity: Rhesus HER2 / ErbB2
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rhesus ErbB2 in WB. Using a DAB detection system, the detection limit for Rhesus ErbB2 is approximately 0.5 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rhesus ErbB2. The detection limit for Rhesus ErbB2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HER2 / ErbB2 Antibody Background

Receptor tyrosine-protein kinase erbB-2, also known as epidermal growth factor receptor-related protein, proto-oncogene Neu, proto-oncogene c-ErbB-2, CD340 and ErbB2, is a single-pass type I membrane protein, cytoplasm protein and nucleus protein which belongs to the protein kinase superfamily, Tyr protein kinase family and EGF receptor subfamily. The ErbB protein family, also known as epidermal growth factor receptor (EGFR) family, is a family of four structurally related receptor tyrosine kinases (ErbB1/EGFR, ErbB2/HER2, ErbB3/HER3, ErbB4/HER4). The gene symbol, ErbB, is derived from the name of a viral oncogene to which these receptors are homologous: Erythroblastic Leukemia Viral Oncogene. Excessive ErbB signaling is associated with the development of a wide variety of types of solid tumor. Insufficient ErbB signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's Disease. Mouse ErbB2/HER2 is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for rat ErbB2/HER2. It binds to the 5'-TCAAATTC-3' sequence in the MT-CO2 promoter and activates its transcription. ErbB2/HER2 is widely expressed in epithelial cells, and amplification and/or overexpression of ErbB2/HER2 has been reported associated with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate and ovarian cancers. ErbB2/HER2 cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. This receptor forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. ErbB2/HER2 mediates signalling pathways which involve mitogen-activated protein kinase and phosphatidylinositol-3 kinase, this receptor plays an key role in development, cell proliferation and differentiation.

References

1. Yamamoto, T. et al., 1986, Nature. 319: 230-34.
2. Lai C., et al., 1991, Neuron 6: 691-704.
3. Gullick W.J., et al., 1992, EMBO J. 11: 43-48.
4. Codony-Seeervat, J. et al., 1999, Cancer Res. 59: 1196-201.
5. Tzahar, E. et al., 1998, Biochim. Biophys. Acta. 1377: 25-37.
6. Kermit, L. et al., 1999, J. Biol. Chem. 274: 5263-66.
7. Nathan J. 2001, J. Biol. Chem. 276: 42153-161.
8. Ying-Xin, Fan. et al., 2008, J. Biol. Chem. 283: 1588-96.