ErbB2 / HER2 / CD340 Antibody ( FITC )

FITC Rabbit Anti-Human ErbB2 / HER2 Antibody

ErbB2 / HER2 Antibody Product Information

• Immunogen: Recombinant human ErbB2 protein ( Catalog#10004-H08H )
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 511
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human ErbB2 / HER2 extracellular domain ( rh ErbB2 ; Catalog#10004-H08H ; NP_004439.2 ; Met 1 - Thr 652 ) and conjugated with FITC under optimum conditions, the unreacted FITC was removed

ErbB2 / HER2 Antibody Usage Guide

• Specificity: Human ErbB2 / HER2 / CD340
• Flow Cytometry:
  

  Flow cytometric analysis of anti-ErbB2 on SK-BR3 cells. SK-BR3 cells were detached using 1X trypsin, washed, then stained with FITC Rabbit anti-Human ErbB2.

  

  Flow cytometric analysis of anti-ErbB2 on HeLa cells. HeLa cells were detached using 1X trypsin, washed, then stained with FITC Rabbit anti-Human ErbB2.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

ErbB2 / HER2 Antibody Background

Receptor tyrosine-protein kinase erbB-2, also known as epidermal growth factor receptor-related protein, proto-oncogene Neu, proto-oncogene c-ErbB-2, CD340 and ErbB2, is a single-pass type I membrane protein, cytoplasm protein and nucleus protein which belongs to the protein kinase superfamily, Tyr protein kinase family and EGF receptor subfamily. The ErbB protein family, also known as epidermal growth factor receptor (EGFR) family, is a family of four structurally related receptor tyrosine kinases (ErbB1/EGFR, ErbB2/HER2, ErbB3/HER3, ErbB4/HER4). The gene symbol, ErbB, is derived from the name of a viral oncogene to which these receptors are homologous: Erythroblastic Leukemia Viral Oncogene. Excessive ErbB signaling is associated with the development of a wide variety of types of solid tumor. Insufficient ErbB signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's Disease. Mouse ErbB2/HER2 is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for rat ErbB2/HER2. It binds to the 5'-TCAAATTC-3' sequence in the MT-CO2 promoter and activates its transcription. ErbB2/HER2 is widely expressed in epithelial cells, and amplification and/or overexpression of ErbB2/HER2 has been reported associated with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate and ovarian cancers. ErbB2/HER2 cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. This receptor forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. ErbB2/HER2 mediates signalling pathways which involve mitogen-activated protein kinase and phosphatidylinositol-3 kinase, this receptor plays an key role in development, cell proliferation and differentiation.

References

1. Yamamoto, T. et al., 1986, Nature.  319: 230-34.
2. Nathan, J. 2001, J. Biol. Chem.  276: 42153-161.
3. Codony-Seeervat, J. et al., 1999, Cancer Res. 59: 1196-201.
4. Tzahar, E. et al., 1998, Biochim. Biophys. Acta. 1377: 25-37.
5. Kermit, L. et al, 1999, J. Biol. Chem. 274: 5263-66.
6. Ying-Xin Fan. et al., 2008, J. Biol. Chem. 283: 1588-96.