All HDAC4 reagents are produced in house and quality controlled, including 2 HDAC4 Antibody, 1 HDAC4 Gene, 1 HDAC4 IPKit, 1 HDAC4 Lysate, 1 HDAC4 Protein. All HDAC4 reagents are ready to use.
Recombinant HDAC4 proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-GST & His, N-cleavage.
HDAC4antibodies are validated with different applications, which are ELISA, WB, ICC/IF, IF, IP.
HDAC4cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each HDAC4 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
HDAC4 (histone deacetylase 4), belongs to class II of the histone deacetylase/acuc/apha family. Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions and by the class to which they belong. HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents. HDAC4 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It does not bind DNA directly, but through transcription factors MEF2C and MEF2D. HDAC4 seems to interact in a multiprotein complex with RbAp48 and HDAC3.