Mouse HCRTR2 Gene cDNA Clone / ORF Clone

HCRTR2 cDNA Clone Datasheet

HCRTR2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_198962.3
• cDNA Size: 1383
• Gene Synonym: OX2R; mOXR2; mOX2aR; mOX2bR; Hcrtr2
• Source: Mouse

HCRTR2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus hypocretin (orexin) receptor 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for four point mutations:718 A/G resulting in the amino acid Ile substitution by Val, 1190 G/A resulting in the amino acid Arg substitution by His, 405 T/G and 534 C/T not causing the amino acid variation.

HCRTR2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HCRTR2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HCRTR2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCAGCA CCAAACTGGA GGATTCCCTC TCTCGTCGCA ACTGGTCATC TGCTTCAGAG
61       CTGAATGAAA CTCAAGAGCC GTTTTTAAAT CCCACGGACT ATGACGACGA GGAATTCCTG
121      CGGTACCTGT GGAGGGAATA CCTACACCCG AAAGAATATG AGTGGGTCCT GATCGCAGGG
181      TATATCATCG TGTTCGTTGT GGCTCTCATC GGGAACGTCC TGGTCTGTGT GGCAGTGTGG
241      AAGAACCACC ACATGAGGAC AGTCACCAAC TACTTCATAG TCAACCTTTC CCTAGCAGAT
301      GTGCTTGTGA CCATCACCTG CCTTCCAGCT ACCCTCGTTG TTGACATCAC TGAGACTTGG
361      TTCTTTGGAC AGTCCCTCTG TAAGGTCATT CCTTATTTAC AGACGGTGTC AGTGTCTGTG
421      TCTGTTCTTA CGTTGAGCTG CATTGCCTTG GACCGATGGT ACGCCATTTG TCACCCTTTG
481      ATGTTCAAGA GCACAGCCAA ACGGGCTCGA AACAGCATCG TTGTCATCTG GATTGTCTCC
541      TGCATCATAA TGATTCCTCA AGCCATTGTC ATGGAGTGCA GCAGCATGCT CCCTGGCCTA
601      GCCAATAAGA CCACCCTCTT TACAGTATGT GATGAACACT GGGGCGGTGA AGTTTACCCA
661      AAGATGTACC ATATCTGCTT CTTTCTGGTG ACATACATGG CACCTCTGTG TCTTATGGTA
721      TTGGCTTATC TCCAAATATT CCGTAAACTC TGGTGCCGAC AGATTCCCGG AACTTCTTCT
781      GTGGTTCAGA GAAAATGGAA GCAGCAGCAG CCGGTTTCTC AGCCCCGGGG GTCCGGACAG
841      CAGAGCAAGG CTCGGATTAG CGCTGTTGCT GCTGAGATAA AGCAGATCCG AGCACGAAGG
901      AAAACAGCCC GGATGCTCAT GGTTGTACTT CTGGTCTTTG CAATTTGCTA TCTACCAATC
961      AGCATCCTCA ATGTGCTAAA GAGAGTATTT GGGATGTTCA CACACACGGA AGACAGAGAG
1021     ACTGTCTATG CTTGGTTCAC TTTTTCTCAT TGGCTTGTAT ATGCCAACAG TGCTGCAAAC
1081     CCAATTATTT ATAATTTTCT TAGTGGAAAA TTTCGAGAGG AATTTAAAGC TGCCTTTTCT
1141     TGTTGTCTTG GGGTTCATCA TCGCCAAGGA GACCGCCTCG CCAGGGGACA CACGAGCACA
1201     GAGAGCAGGA AGTCCCTGAC CACACAGATC AGCAACTTTG ACAATGTATC AAAACTCTCA
1261     GAGCACGTGG TGCTCACCAG CATAAGCACA CTCCCAGCAG CCAACGGGGC AGGACCGCTT
1321     CAAAACTGGT ATCTACAGCA GGGAGTACCA TCTTCACTCC TGTCCACCTG GCTGGAGGTC
1381     TGA

 

Quality Control:cDNA sequencing results is available upon request.