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HBP1 Antibody, Rabbit PAb

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    Human HBP1 Antibody Product Information
    Immunogen:Recombinant Human HBP1 protein (Catalog#14127-H09E)
    Clone ID:
    Ig Type:Rabbit IgG
    Concentration:
    Endotoxin:
    Formulation:0.2 μm filtered solution in PBS with 5% trehalose
    Preparation:Produced in rabbits immunized with purified, recombinant Human HBP1 (rh HBP1; Catalog#14127-H09E; O60381-1; Pro208-Phe345). Total IgG was purified by Protein A affinity chromatography.
    Other HBP1 Antibody Products

    Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution

    Lane A: NIH3T3 Whole Cell Lysate

    Lane B: Hela Whole Cell Lysate

    Lysates/proteins at 30 μg per lane.

    Secondary

    Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size:15 kDa

    Observed band size:17 kDa

    HBP1 Background

    HBP1 is a sequence-specific DNA-binding transcription factor. It is involved in many biological processes. It was reported that HBP1 binds to p16(INK4A) promoter and activates p16(INK4A) expression. We found that trichostatin A (TSA), an inhibitor of HDAC (histone deacetylase), induces p16(INK4A) expression in an HBP1-dependent manner. HBP1 activates or represses the expression of some specific genes during cell growth and differentiation. HBP1 was acetylated by p300/CBP in two regions: repression domain (K297/305/307) and P domain (K171/419). HBP1 acetylation after TSA treatment was confirmed by immunoprecipitation assay. HBP1 interacted with histone acetyltransferase p300 and CREB-binding protein (CBP) and also recruited p300/CBP to p16(INK4A) promoter. HBP1 acetylation at K419 plays an important role in HBP1-induced p16(INK4A) expression.

    Human HBP1 References
  • Tevosian SG. et al., 1997, Genes Dev. 11 (3): 383-96.
  • Lavender P. et al., 1997, Oncogene. 14 (22): 2721-8.
  • Swanson. et al., 2004, Nat Struct Mol Biol. 11 (8): 738-46.
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    Size / Price
    Catalog: 14127-RP01-200
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"