HAO1 Antibody

HAO1 Antibody Datasheet

HAO1 Antibody Product Information

• Immunogen: Recombinant Human HAO1 / GOX1 protein (Catalog#12076-H07E)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human HAO1 (rh HAO1; Catalog#12076-H07E; NP_060015.1; Leu 2-Ile 370).

HAO1 Antibody Usage Guide

• Specificity: Human HAO1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human HAO-1 in WB. Using a DAB detection system, the detection limit for Human HAO-1 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HAO-1. The detection limit for Human HAO-1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HAO1 Antibody Background

Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1 and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family. HAO1 / GOX1 has 2-hydroxyacid oxidase activity. It is most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. HAO1 / GOX1 is a liver-specific peroxisomal enzyme that oxidizes glycolate to glyoxylate with concomitant production of H2O2. In Hao1 messenger RNA (mRNA), an iron-responsive element (IRE) homologous to the sequence recognized by iron regulatory proteins (IRP), key regulators of iron homeostasis, is present. Mammalian HAO1 / GOX1 is a peroxisomal protein and that the C-terminal sequence SKI acts as the targeting signal. Down-regulation of HAO1 / GOX1 expression during oxidative stress may provide a mechanism to prevent excessive H2O2 formation in liver peroxisomes and may represent the prototype of a poorly recognized but potentially relevant response to oxidative injury involving down-regulation of ROS-producing enzymes.

References

1. Jones J.M.et al., 2000, J. Biol. Chem. 275: 12590-7.
2. Recalcati,S. et al., 2001, J Cell Sci. 114 (Pt 9): 1625-9.
3. Recalcati,S. et al., 2003, Hepatology. 38 (5): 1159-66.
4. Murray M.S.et al., 2008, Biochemistry 47: 2439-49.
5. Bourhis J.M.et al., 2009, Acta Crystallogr. F 65: 1246-53.