Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase cDNA Clone Datasheet

Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase cDNA Clone Product Information

• cDNA Size: 1404
• Gene Synonym: NA, Neuraminidase
• Source: Influenza Virus

Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: pGEM-T Vector

Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H9N2 (A/Hong Kong/1073/99) Neuraminidase cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATCCAA ATCAAAAGAT AATAGCACTT GGCTCTGTTT CTATAACTAT TGCGACAATA
61       TGTTTACTCA TGCAGATTGC CATCTTAGCA ACGACTATGA CACTACATTT CAATGAATGT
121      ACCAACCCAT CGAACAATCA AGCAGTGCCA TGTGAACCAA TCATAATAGA AAGGAACATA
181      ACAGAGATAG TGCATTTGAA TAATACTACC ATAGAGAAGG AAAGTTGTCC TAAAGTAGCA
241      GAATACAAGA ATTGGTCAAA ACCACAATGT CAAATCACAG GGTTCGCTCC TTTCTCCAAG
301      GACAATTCAA TTAGGCTCTC CGCAGGTGGA GATATTTGGG TGACAAGAGA ACCTTATGTA
361      TCGTGCGGTC TTGGTAAATG TTATCAATTT GCACTTGGGC AGGGAACCAC TTTGAACAAC
421      AAACACTCAA ATGGCACAAT ACATGATAGG AGTCCCCATA GAACCCTTTT AATGAACGAG
481      TTGGGTGTTC CATTTCATTT GGGAACCAAA CAAGTGTGCA TAGCATGGTC CAGCTCAAGC
541      TGCCATGATG GGAAAGCATG GTTACATGTT TGTGTCACTG GGGATGATAG AAATGCAACG
601      GCTAGCATCA TTTATGATGG GATGCTTACT GACAGTATTG GTTCATGGTC TAAAAACATC
661      CTCAGAACTC AGGAGTCAGA ATGCGTTTGC ATCAATGGAA CCTGTACAGT AGTAATGACT
721      GATGGAAGTG CATCAGGAAG GGCTGACACT AAAATACTAT TTATTAGAGA GGGGAAAATT
781      GTACACATTG GCCCATTGTC AGGAAGTGCT CAGCATGTGG AGGAATGCTC CTGTTACCCC
841      CGATATCCAG AAGTTAGATG TGTTTGCAGA GACAATTGGA AGGGATCCAA TAGGCCCGTT
901      CTATATATAA ATGTGGCAGA TTATAGTGTT GATTCCAGTT ATGTGTGCTC AGGACTTGTT
961      GGCGACACAC CAAGAAATGA TGATAGTTCT AGCAGCTCTA ACTGCAGAGA TCCTAATAAC
1021     GAGAGAGGGG GCCCAGGAGT AAAAGGGTGG GCCTTTGACA ATGGAAATGA CGTTTGGATG
1081     GGAAGAACAA TCAAAAAGGA TTCGCGCTCA GGTTATGAGA CTTTCAGGGT CGTTGGTGGT
1141     TGGACCACTG CTAATTCCAA GTCACAGATA AATAGACAAG TCATAGTTGA CAGTGATAAC
1201     TGGTCTGGGT ATTCTGGTAT CTTCTCTGTT GAAGGCAAAA CCTGCATCAA CAGGTGTTTT
1261     TACGTGGAGT TGATAAGAGG AAGACCACAG GAGACTAGGG TGTGGTGGAC TTCAAATAGC
1321     ATCATTGTAT TTTGTGGAAC TTCAGGTACC TATGGAACAG GCTCATGGCC TGATGGGGCG
1381     AATATCAATT TCATGTCTAT ATAA

 

Quality Control:cDNA sequencing results is available upon request.