Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin

Influenza A Virus H9N2 ( A/Hong Kong/1073/99 ) HA Protein Datasheet

H9N2 (A/Hong Kong/1073/99) HA Protein Product Information

• Synonym: HA
• Protein Construction: A DNA sequence encoding the extracellular domain of Influenza A virus H9N2 hemagglutinin (Influenza A virus ( A/Hong Kong/1073/99 (H9N2) ) (NP_859037.1)(Met 1-Lys 523)(HA1+HA2, uncleaved) was fused with a polyhistidine tag at the C-terminus.
• Source: Influenza A Virus H9N2
• Expression Host: Human Cells

H9N2 (A/Hong Kong/1073/99) HA Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 19
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus H9N2 ( A/Hong Kong/1073/99 ) comprises 516 amino acids with the predicted molecular mass of 58 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: H9N2 HA protein

H9N2 (A/Hong Kong/1073/99) HA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H9N2 (A/Hong Kong/1073/99) HA Protein Description

Hemagglutinin (HA) is a single-pass type I  integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence. The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Hemagglutinin Proteins
• H9N2 Hemagglutinin & Antibody
• A/Hong Kong/1073/99

H9N2 (A/Hong Kong/1073/99) HA Protein Related Products & Topics

• H9N2 HA Protein Related Areas:
• Proteins:
• Antibodies:

References

1. Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.
2. Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.
3. Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.
4. Gething, M-J. et al., 1980, Nature. 287: 301-306.
5. Lin, Y.P. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 9654-9658.