Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin partial cDNA Clone / ORF Clone

Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin cDNA Clone Datasheet

Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin cDNA Clone Product Information

• cDNA Size: 1610
• Gene Synonym: HA, Hemagglutinin
• Source: Influenza Virus

Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin DNA.
• Vector: pGEM-T Vector

Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGCTG TACCACTAAT AACTATACTA CTAGTAGTAA CAGCAAGCAA TGCAGACAAA
61       ATCTGCATCG GCTACCAATC AACAAACTCC ACAGAAACCG TAGACACGCT AACAGAAAAC
121      AATGTTCCTG TGACACATGC CAAAGAATTG CTCCACACAG AGCACAATGG GATGCTGTGT
181      GCAACAAATC TGGGACGTCC TCTTATTCTA GACACTTGCA CCATTGAAGG ACTGATCTAT
241      GGCAACCCTT CTTGTGATCT ACTGTTGGGA GGAAGAGAAT GGTCCTACAT CGTCGAAAGA
301      CCATCGGCTG TTAATGGAAT GTGTTACCCC GGGAATGTAG AAAACCTAGA GGAACTAAGG
361      TCATTTTTTA GTTCTGCTAG TTCCTACCAA AGAATCCAGA TCTTTCCAGA CACAATCTGG
421      AATGTGTCTT ACAGTGGAAC AAGCAAAGCA TGTTCAGATT CATTCTACAG GAGCATGAGA
481      TGGTTGACTC AAAAGAACAA CGCTTACCCT ATTCAAGACG CCCAATACAC AAATAATAGA
541      GGAAAGAGCA TTCTTTTCAT GTGGGGCATA AATCACCCAC CTACCGATAC TGCACAGACA
601      AATCTGTACA CAAGGACTGA CACAACAACA AGTGTGGCAA CAGAAGATAT AAATAGGACC
661      TTCAAACCAG TGATAGGGCC AAGGCCCCTT GTCAATGGTC TGCAGGGAAG AATTGATTAT
721      TATTGGTCGG TATTGAAACC AGGTCAGACA TTGCGAGTAA GATCCAATGG GAATCTAATC
781      GCTCCATGGT ATGGGCACAT TCTTTCAGGA GAGAGCCACG GAAGAATCCT GAAGACTGAT
841      TTAAACAGTG GTAGCTGTGT AGTGCAATGT CAAACAGAAA GAGGTGGCTT AAATACTACT
901      TTGCCATTCC ACAATGTCAG TAAATATGCA TTTGGAAACT GCCCAAAATA TGTTGGAGTA
961      AAGAGTCTCA AACTGGCAGT TGGTCTGAGG AATGTGCCTG CTAGATCAAG TAGAGGACTA
1021     TTTGGGGCCA TAGCTGGATT CATAGAGGGA GGTTGGTCAG GGCTGGTCGC TGGTTGGTAT
1081     GGGTTCCAGC ATTCAAATGA TCAAGGGGTT GGTATAGCTG CAGATAGAGA CTCAACTCAA
1141     AGGGCAATTG ACAAAATAAC GTCCAAAGTG AATAATATAG TCGATAAAAT GAACAAGCAA
1201     TATGAAATTA TTGATCATGA ATTCAGCGAG GTTGAAAATA GACTCAATAT GATCAATAAT
1261     AAGATTGATG ACCAGATACA AGACATATGG GCATATAACG CTGAATTGCT AGTGCTGCTT
1321     GAAAACCAGA AAACACTCGA TGAGCATGAT GCGAATGTAA ACAATCTATA TAACAAAGTG
1381     AAGAGGGCAC TGGGTTCCAA TGCAATGGAA GATGGGAAAG GATGTTTCGA GCTATACCAT
1441     AAATGTGATG ATCAGTGCAT GGAGACAATT CGGAACGGGA CCTATAACAG GAGGAAGTAT
1501     AAAGAGGAAT CAAGACTAGA AAGACAGAAA ATAGAAGGGG TCAAGCTGGA ATCTGAAGGA
1561     ACTTACAAAA TCCTCACCAT TTATTCGACT GTCGCCTCAT CTCTTGTAAA

 

Quality Control:cDNA sequencing results is available upon request.