Influenza A H8N4 (A/pintail duck/Alberta/114/1979) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H8N4 (A/pintail duck/Alberta/114/1979) cDNA Clone Datasheet

Influenza A H8N4 (A/pintail duck/Alberta/114/1979) cDNA Clone Product Information

• cDNA Size: 1723
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H8N4 (A/pintail duck/Alberta/114/1979) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H8N4 (A/pintail duck/Alberta/114/1979) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H8N4 (A/pintail duck/Alberta/114/1979) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H8N4 (A/pintail duck/Alberta/114/1979) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H8N4 (A/pintail duck/Alberta/114/1979) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGGA GAAACTGATT GTGATTGCTA TGCTGCTGGC AAGCACCAAT
61       GCCTATGACA GGATTTGTAT TGGCTACCAG AGCAACAACA GCACAGACAC AGTGAACACC
121      CTGATTGAAC AGAATGTGCC TGTGACCCAG ACAATGGAAC TGGTGGAGAC AGAGAAACAC
181      CCTGCCTACT GTAACACAGA CCTGGGAGCC CCATTGGAAC TGAGGGACTG TAAGATTGAG
241      GCTGTGATTT ATGGCAACCC AAAGTGTGAC ATCCACCTGA AAGACCAGGG CTGGTCCTAC
301      ATTGTGGAGA GACCATCTGC CCCTGAGGGG ATGTGTTACC CTGGCAGCAT TGAGAACTTG
361      GAGGAACTGA GGTTTGTGTT CTCCTCTGCT GCCTCCTACA AGAGGATTAG ACTGTTTGAC
421      TACAGCAGGT GGAATGTGAC CAGGTCTGGC ACCAGCAAGG CTTGTAATGC CAGCACAGGA
481      GGACAATCCT TCTACAGGAG CATCAACTGG CTGACCAAGA AGAAGCCTGA CACCTATGAC
541      TTCAATGAGG GCACCTATGT GAACAATGAG GATGGAGACA TCATCTTCCT GTGGGGCATC
601      CACCACCCTC CTGACACCAA GGAACAGACC ACCCTCTACA AGAATGCCAA CACCCTGACC
661      TCTGTGACCA CCAACACCAT CAACAGGAAC TTCCAACCAA ACATTGGACC AAGACCACTG
721      GTGAGGGGAC AACAGGGCAG GATGGACTAC TACTGGGGCA TCCTGAAAAG GGGAGAGACC
781      CTGAAAATCA GGACCAATGG CAACCTGATT GCCCCTGAGT TTGGCTACCT GCTGAAAGGA
841      GAGTCTCATG GCAGGATTAT CCAGAATGAG GACATCCCAA TTGGCAACTG TAACACCAAG
901      TGTCAGACCT ATGCTGGAGC CATCAACTCC AGCAAGCCAT TCCAGAATGC CAGCAGACAC
961      TATATGGGAG AGTGTCCAAA ATATGTGAAG AAGGCATCCC TGAGACTGGC TGTGGGACTG
1021     AGGAACACAC CATCTGTGGA ACCAAGGGGA CTGTTTGGAG CCATTGCTGG CTTCATTGAG
1081     GGAGGCTGGT CTGGGATGAT TGATGGCTGG TATGGCTTCC ACCACAGCAA CTCTGAGGGC
1141     ACAGGGATGG CTGCTGACCA GAAGAGCACC CAGGAGGCTA TTGACAAGAT TACCAACAAG
1201     GTGAACAACA TTGTGGACAA GATGAACAGG GAGTTTGAGG TGGTGAACCA TGAGTTCTCT
1261     GAGGTGGAGA AGAGGATAAA TATGATAAAT GACAAGATTG ATGACCAGAT TGAGGACCTG
1321     TGGGCTTACA ATGCTGAACT GCTGGTGCTG TTGGAGAACC AGAAGACCCT GGATGAACAT
1381     GACAGCAATG TGAAGAACCT GTTTGATGAG GTGAAGAGGA GACTGTCTGC CAATGCCATT
1441     GATGCTGGCA ATGGCTGTTT TGACATCCTG CACAAGTGTG ACAATGAGTG TATGGAGACC
1501     ATCAAGAATG GCACCTACAA CCACAAGGAA TATGAGGAGG AGGCTAAATT GGAGAGGAGC
1561     AAGATAAATG GAGTGAAATT GGAGGAGAAC ACCACCTACA AGATTCTGAG CATCTACAGC
1621     ACAGTGGCTG CCTCCCTGTG TCTGGCTATC CTGATTGCTG GAGGACTGAT TCTGGGGATG
1681     CAGAATGGCT CCTGTAGGTG TATGTTCTGT ATTTAAATCT AGA

 

Quality Control:cDNA sequencing results is available upon request.