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Influenza A H7N7 Hemagglutinin (HA) ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK11082 |
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Influenza A H7N7 HA / Hemagglutinin ELISA Pair Set
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Order or Inquire for Influenza A H7N7 HA ELISA kit |
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High affinityInfluenza A H7N7 HA ELISA kit |
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Detection limit - 46.8 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Influenza A H7N7 HA / Hemagglutinin ELISA Pair Set Related Products & Topics
Background
Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion “fusion peptide”. After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle’s own membrane and the endosomal membrane.
H7N7 is a subtype of Influenza virus A. It can infect humans, birds, pigs, seals, and horses in the wild and has infected mice in laboratory studies. H7N7 represents a pandemic threat because influenza H7N7 viruses do not commonly infect humans, there is little or no antibody protection against these viruses in the human population. If an avian or other animal influenza virus of H7N7 is able to infect people, cause illness, and spread efficiently from person to person, an influenza pandemic could begin. In 2003 in the Netherlands 89 people were confirmed to have the H7N7 influenza virus infection following an outbreak in poultry on several farms. One death was recorded. Antibodies were found in over half of 500 people tested according to the final official report by the Dutch government. Final analysis of Dutch avian influenza outbreaks reveals much higher levels of transmission to humans than previously thought.
