Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) cDNA Clone Datasheet

Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) cDNA Clone Product Information

• cDNA Size: 1723
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGAT TGCCATCATC ATCATTGCCA TCCTGGCTGC TGCTGGCAGG
61       TCTGACAAGA TTTGTATTGG CTACCATGCC AACAACAGCA CCACCCAGGT GGACACCATC
121      TTGGAGAAGA ATGTGACAGT GACCCACTCT GTGGAACTGT TGGAGAACCA GAAGGAGGAG
181      AGGTTCTGTA AGATTCTGAA CAAGGCTCCA CTGGACCTGA GGGGCTGTAC CATTGAGGGC
241      TGGATTCTGG GCAACCCACA GTGTGACCTG CTGCTGGGAG ACCAGTCCTG GTCCTACATT
301      GTGGAGAGAC CAACAGCCCA GAATGGCATC TGTTACCCTG GAGCCCTGAA TGAGGTGGAG
361      GAACTGAAAG CCCTGATTGG CTCTGGAGAG AGGGTGGAGA GGTTTGAGAT GTTTCCAAAG
421      AGCACCTGGA CAGGAGTGGA CACCTCCTCT GGAGTGACCA AGGCTTGTCC ATACAACTCT
481      GGCTCCTCCT TCTACAGGAA CCTGCTGTGG ATTATCAAGA CCAAGTCTGC TGCCTACCCT
541      GTGATTAAGG GCACCTACAA CAACACAGGC AGCCAACCAA TCCTCTACTT CTGGGGAGTG
601      CATCACCCTC CTGACACCAA TGAACAGAAC ACCCTCTATG GCTCTGGAGA CAGATATGTG
661      AGGATGGGCA CAGAGAGTAT GAACTTTGCC AAGAGCCCTG AGATTGCTGC CAGACCTGCT
721      GTGAATGGAC AAAGGGGCAG GATTGACTAC TACTGGTCTG TGCTGAAACC TGGAGAGACC
781      CTGAATGTGG AGAGCAATGG CAACCTGATT GCCCCATGGT ATGCCTACAA GTTTGTGAGC
841      ACCAACAACA AGGGAGCCAT CTTCAAGAGC AACCTGCCAA TTGAGAACTG TGATGCCACT
901      TGTCAGACCA TTGCTGGAGT GCTGAGGACC AACAAGACCT TCCAGAATGT GAGCCCACTG
961      TGGATTGGAG AGTGTCCAAA ATATGTGAAG TCTGAGTCCC TGAGACTGGC TACAGGACTG
1021     AGGAATGTGC CACAGATTGA GACCAGGGGA CTGTTTGGAG CCATTGCTGG CTTCATTGAG
1081     GGAGGCTGGA CAGGGATGAT TGATGGCTGG TATGGCTACC ACCATGAGAA CAGCCAGGGC
1141     TCTGGCTATG CTGCTGACAA GGAGAGCACC CAGAAGGCTA TTGATGGCAT CACCAACAAG
1201     GTGAACAGCA TCATTGACAA GATGAACACC CAGTTTGAGG CTGTGGACCA TGAGTTCAGC
1261     AACTTGGAGA GGAGGATTGA CAACCTGAAC AAGAGGATGG AGGATGGCTT CCTGGATGTG
1321     TGGACCTACA ATGCTGAACT GCTGGTGCTG TTGGAGAATG AGAGGACCCT GGACCTCCAT
1381     GATGCCAATG TGAAGAACCT CTATGAGAAG GTGAAGAGCC AACTTAGGGA CAATGCCAAT
1441     GACCTGGGCA ATGGCTGTTT TGAGTTCTGG CACAAGTGTG ACAATGAGTG TATTGAGTCT
1501     GTGAAGAATG GCACCTATGA CTACCCAAAA TACCAGGATG AGAGCAAACT GAACAGACAG
1561     GAGATTGAGT CTGTGAAACT GGACAACCTG GGAGTCTACC AGATTCTGGC TATCTACAGC
1621     ACAGTGTCCT CCTCCCTGGT GCTGGTGGGA CTGATTATTG CTATGGGACT GTGGATGTGT
1681     AGCAATGGCA GTATGCAGTG TAGGATTTGT ATTTAAATCT AGA

 

Quality Control:cDNA sequencing results is available upon request.