Influenza A H5N8 (A/duck/NY/191255-59/02) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H5N8 (A/duck/NY/191255-59/02) cDNA Clone Datasheet

Influenza A H5N8 (A/duck/NY/191255-59/02) cDNA Clone Product Information

• cDNA Size: 1717
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H5N8 (A/duck/NY/191255-59/02) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H5N8 (A/duck/NY/191255-59/02) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XbaI

Influenza A H5N8 (A/duck/NY/191255-59/02) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H5N8 (A/duck/NY/191255-59/02) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H5N8 (A/duck/NY/191255-59/02) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGGA GAAGATTGTG CTGCTGCTGG CTATCATCTC CCTGGTGAAG
61       TCTGACCAGA TTTGTATTGG CTACCATGCC AACAACAGCA CAGAACAGGT GGACACCATA
121      ATGGAGAAGA ATGTGACAGT GACCCATGCC CAGGACATCT TGGAGAAGGA ACACAATGGC
181      AAACTGTGTT CCCTGAAAGG AGTGAGACCA CTGATTCTGA AAGACTGTTC TGTGGCTGGC
241      TGGCTGCTGG GCAACCCTAT GTGTGATGAG TTCCTGAATG TGCCTGAGTG GTCCTACATT
301      GTGGAGAAGG ACAACCCTGT GAATGGACTG TGTTACCCTG GAGACTTCAA TGACTATGAG
361      GAACTGAAAC ACCTGATGAG TAGCACCAAC CACTTTGAGA AGATTCAGAT TATCCCAAGG
421      TCCTCCTGGA GCAACCATGA TGCCTCCTCT GGAGTGTCCT CTGCCTGTCC ATACAATGGC
481      AGGTCCTCCT TCTTCAGGAA TGTGGTGTGG CTGATTAAGA AGAACAATGC CTACCCAACC
541      ATCAAGAGGA CCTACAACAA CACCAATGTG GAGGACCTGC TGATTCTGTG GGGCATCCAC
601      CACCCAAATG ATGCTGCTGA ACAGACCAAA CTCTACCAGA ACAGCAACAC CTATGTGTCT
661      GTGGGCACCA GCACCCTGAA CCAGAGGAGC ATCCCTGAGA TTGCCACCAG ACCAAAGGTG
721      AATGGACAAT CTGGCAGGAT GGAGTTCTTC TGGACCATCC TGAGACCAAA TGATGCCATC
781      TCCTTTGAGA GCAATGGCAA CTTCATTGCC CCTGAATATG CCTACAAGAT TGTGAAGAGG
841      GGAGACTCTG CCATAATGAA GTCTGAATTG GAATATGGCA ACTGTGACAC CAAGTGTCAG
901      ACACCTATGG GAGCCATCAA CTCCAGTATG CCATTCCACA ATGTGCATCC ACTGACCATT
961      GGAGAGTGTC CAAAATATGT GAAGTCTGAC AAACTGGTGC TGGCTACAGG ACTGAGGAAT
1021     GTGCCACAGA GGGAGACCAG GGGACTGTTT GGAGCCATTG CTGGCTTCAT TGAGGGAGGC
1081     TGGCAGGGGA TGGTGGATGG CTGGTATGGC TACCACCACA GCAATGAACA GGGCTCTGGC
1141     TATGCTGCTG ACAAGGAGAG CACCCAGAAG GCTATTGATG GCATCACCAA CAAGGTGAAC
1201     AGCATCATTG ACAAGATGAA CACCCAGTTT GAGGCTGTGG GCAAGGAGTT CAGCAACTTG
1261     GAGAGGAGGA TTGAGAACCT GAACAAGAAG ATGGAGGATG GCTTCCTGGA TGTGTGGACC
1321     TACAATGCTG AACTGCTGGT GCTGATGGAG AATGAGAGGA CCCTGGACTT CCATGACAGC
1381     AATGTGAAGA ACCTCTATGA CAAGGTGAGA CTCCAACTTA GGGACAATGC CAAGGAACTG
1441     GGCAATGGCT GTTTTGAGTT CTACCACAAG TGTGACAATG AGTGTATGGA GTCTGTGAGG
1501     AATGGCACCT ATGACTACCC ACAATACTCT GAGGAGAGCA GACTGAACAG GGAGGAGATT
1561     GATGGAGTGA AATTGGAGAG TATGGGCACC TACCAGATTC TGAGCATCTA CAGCACAGTG
1621     GCATCCTCCC TGGCTCTGGC TATAATGATT GCTGGACTGT CCTTCTGGAT GTGTAGCAAT
1681     GGCTCCCTCC AATGTAGGAT TTGTATTTAA ATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.