Influenza A H5N2 HA / Hemagglutinin Antibody (Antigen Affinity Purified)

H5N2 HA / Hemagglutinin Antibody ( Antigen Affinity Purified ) Datasheet

H5N2 HA / Hemagglutinin Antibody Product Information

• Immunogen: Recombinant H5N2 (A/American green-winge teal/California/ HKWF609/07) HA / Hemagglutinin protein (Catalog#11699-V08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin (Catalog#11699-V08H; ACF47563.1; Met 1-Gln 527). H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin specific IgG was purified by H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin affinity chromatography.

H5N2 HA / Hemagglutinin Antibody Usage Guide

• Specificity: H5N2 (A/American green-winge teal/California/HKWF609/07) HA / Hemagglutinin
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect H5N2 (A/American green-winged teal/California/HKWF609/07) HA in WB. Using a DAB detection system, the detection limit for H5N2 (A/American green-winged teal/California/HKWF609/07) HA is approximately 4 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect H5N2 (A/American green-winged teal/California/HKWF609/07) HA. The detection limit for H5N2 (A/American green-winged teal/California/HKWF609/07) HA is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

H5N2 HA / Hemagglutinin Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). H5N2is a subtype of the speciesInfluenzavirus A. Low pathogenic avian inluenza H5N2 virus in poultry gained accentuated virulence in the United States and Mexico. A highly pathogenic strain of H5N2 caused flu outbreaks with significant spread to numerous farms, resulting in great economic losses in 1983 in Pennsylvania, USA in chickens and turkeys, in 1994 in Mexico in chickens and a minor outbreak in 1997 in Italy in chickens. In 2006, an H5N2 outbreak on a single farm in South Africa resulted in the destruction of all its sixty ostriches. The strain was similar to the one that caused outbreaks in South Africa 2004/2005. In 2007, a low-pathogenic strain of H5N2 was found in samples collected from 25,000 turkeys in Pendleton County, West Virginia in a routine testing prior to their slaughter. In late 2007 (December 21), an H5N2 outbreak was found in the Dominican Republic, in a Suburb of Higuey City, on the eastern side of the island. In May and June 2008, there were three outbreaks of low-pathogenic H5N2 avian flu in birds at three locations in the central, northern, and southern parts of Haiti. "The outbreaks began on May 20 and appeared to be ongoing", as of June 17, 2008. In China, inactivated H5N2 has been used as a vaccine for H5N1. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

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