Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin Gene cDNA Clone / ORF Clone (Codon Optimized)

Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin cDNA Clone Datasheet

Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin cDNA Clone Product Information

• cDNA Size: 1714
• Gene Synonym: HA, Hemagglutinin
• Source: Influenza Virus

Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H5N1 (A/goose/Guangdong/3/96) Hemagglutinin cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAGGA TTGTGCTGCT GCTGGCTATT GTGTCCCTGG TGAAGTCTGA CCAGATTTGT
61       ATTGGCTACC ATGCCAACAA CAGCACAGAA CAGGTGGACA CCATAATGGA GAAGAATGTG
121      ACAGTGACCC ATGCCCAGGA CATCTTGGAG AAGACCCACA ATGGCAAACT GTGTGACCTG
181      AATGGAGTGA AGCCACTGAT TCTGAGGGAC TGTTCTGTGG CTGGCTGGCT GCTGGGCAAC
241      CCTATGTGTG ATGAGTTCAT CAATGTGCCT GAGTGGTCCT ACATTGTGGA GAAGGCAAGC
301      CCTGCCAATG ACCTGTGTTA CCCTGGAGAC TTCAATGACT ATGAGGAACT GAAACACCTG
361      CTGAGCAGGA CAAACCACTT TGAGAAGATT CAGATTATCC CAAAGTCCTC CTGGAGCAAC
421      CATGATGCCT CCAGCGGTGT GTCCTCTGCC TGTCCATATC ATGGCAGGTC CTCCTTCTTC
481      AGGAATGTGG TGTGGCTGAT TAAGAAGAAC AGCGCCTACC CAACCATCAA GAGGTCCTAC
541      AACAACACCA ACCAGGAGGA CCTGCTGGTG CTGTGGGGCA TCCACCACCC AAATGATGCT
601      GCTGAACAGA CCAAACTCTA CCAGAACCCA ACCACCTACA TCTCTGTGGG CACCAGCACC
661      CTGAACCAGA GACTGGTGCC TGAGATTGCC ACCAGACCAA AGGTGAATGG ACAATCTGGC
721      AGGATGGAGT TCTTCTGGAC CATCCTGAAA CCAAATGATG CCATCAACTT TGAGAGCAAT
781      GGCAACTTCA TTGCCCCTGA ATATGCCTAC AAGATTGTGA AGAAGGGGGA CTCTGCCATA
841      ATGAAGTCTG AATTGGAATA TGGCAACTGT AACACCAAGT GTCAGACACC TATGGGAGCC
901      ATCAACTCCA GTATGCCATT CCACAACATC CACCCACTGA CCATTGGAGA GTGTCCAAAA
961      TATGTGAAGA GCAACAGACT GGTGCTGGCT ACAGGACTGA GGAACACACC ACAGAGAGAG
1021     AGAAGGAGAA AGAAGAGGGG ACTGTTTGGA GCCATTGCTG GCTTCATTGA GGGAGGCTGG
1081     CAGGGGATGG TGGATGGCTG GTATGGCTAC CACCACAGCA ATGAACAGGG CTCTGGCTAT
1141     GCTGCTGACA AGGAGAGCAC CCAGAAGGCT ATTGATGGAG TGACCAACAA GGTGAACAGC
1201     ATCATTGACA AGATGAACAC CCAGTTTGAG GCTGTGGGCA GGGAGTTCAA CAACTTGGAG
1261     AGGAGGATTG AGAACCTGAA CAAGCAGATG GAGGATGGCT TCCTGGATGT GTGGACCTAC
1321     AATGCTGAAC TGCTGGTGCT GATGGAGAAT GAGAGGACCC TGGACTTCCA TGACAGCAAT
1381     GTGAAGAACC TCTATGACAA GGTGAGACTC CAACTTAGGG ACAATGCCAA GGAACTGGGC
1441     AATGGCTGTT TTGAGTTCTA CCACAAGTGT GGCAATGAGT GTATGGAGTC TGTGAAGAAT
1501     GGCACCTATG ACTACCCACA ATACTCTGAG GAGGCAAGAC TGAACAGGGA GGAGATTTCT
1561     GGAGTGAAAT TGGAGAGTAT GGGCACCTAC CAGATTCTGC CCATCTACAG CACAGTGGCA
1621     TCCTCCCTGG CTCTGGCTAT AATGGTGGCT GGACTGTCCC TGTGGATGTG TAGCAATGGC
1681     TCCCTCCAAT GTAGGATTTG TATCTAAATC TAGA

 

Quality Control:cDNA sequencing results is available upon request.