Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin Gene cDNA Clone / ORF Clone (Codon Optimized)

Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin cDNA Clone Datasheet

Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin cDNA Clone Product Information

• cDNA Size: 1704
• Gene Synonym: HA, Hemagglutinin
• Source: Influenza Virus

Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H5N1 (A/Vietnam/UT31413II/2008) Hemagglutinin cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAAAA TAGTGCTTCT TCTTGCAATA ATCAGCCTTG TTAAAAGTGA TCAGATTTGC
61       ATTGGTTACC ATGCAAACAA CTCGACAGAG CAGGTTGACA CAATAATGGA AAAGAACGTT
121      ACTGTTACAC ATGCCCAAGA CATACTGGAA AAGACACACA ACGGGAAGCT CTGCGATCTA
181      GATGGAGTGA AGCCTCTGAT TTTAAGAGAT TGTAGTGTAG CTGGATGGCT CCTCGGAAAC
241      CCAATGTGTG ACGAATTCAT CAATGTGCCG GAATGGTCTT ACATAGTGGA GAAGGCCAAC
301      CCAGCCAATG ACCTCTGTTA CCCAGGGAAT TTCAACGACT ATGAAGAACT GAAACACCTA
361      TTGAGCAGAA TAAACCATTT TGAGAAAATT CAGATCATCC CCAAAAGTTC TTGGTCCGAT
421      CATGAAGCCT CATCAGGGGT GAGCTCAGCA TGTCCATACC AGGGAGTGCC CTCCTTTTTC
481      AGAAATGTGG TATGGCTTAT CAAAAAGAAC AATACATACC CAACAATAAA GAGAAGCTAC
541      AATAATACCA ACCAGGAAGA TCTTTTGATA CTGTGGGGGA TTCATCATTC TAATGATGCG
601      GCAGAGCAGA CAAAGCTCTA TCAAAACCCA ACCACCTATA TTTCCGTTGG GACATCAACA
661      CTAAACCAGA GATTGGTACC AAAAATAGCT ACTAGATCCA AAGTAAACGG GCAAAGTGGA
721      AGGATGGATT TCTTCTGGAC AATTTTAAAA CCGAATGATG CAATCAACTT CGAGAGTAAT
781      GGAAATTTCA TTGCTCCAGA ATATGCATAC AAAATTGTCA AGAAAGGGGA CTCAGCAATT
841      ATGAAAAGTG AAGTGGAATA TGGTAACTGC AACACAAAGT GTCAAACTCC AATAGGGGCG
901      ATAAACTCTA GTATGCCATT CCACAACATA CACCCTCTCA CCATCGGGGA ATGCCCCAAA
961      TATGTGAAAT CAAACAAATT AGTCCTTGCG ACTGGGCTCA GAAATAGTCC TCTAAGAGAA
1021     AGAAGAAGAA AAAGAGGACT ATTTGGAGCT ATAGCAGGGT TTATAGAGGG AGGATGGCAG
1081     GGAATGGTAG ATGGTTGGTA TGGGTTCCAC CATAGCAATG AGCAGGGGAG TGGGTACGCT
1141     GCAGACAAAG AATCCACTCA AAAGGCAATA GATGGAGTCA CCAATAAGGT CAACTCGATC
1201     ATTGACAAAA TGAACACTCA GTTTGAGGCC GTTGGAAGGG AATTTAATAA CTTAGAAAGG
1261     AGAATAGAGA ATTTAAACAA GAAAATGGAA GACGGATTCC TAGATGTCTG GACTTATAAT
1321     GCTGAACTTC TGGTTCTCAT GGAAAATGAG AGAACTCTAG ACTTCCATGA TTCAAATGTC
1381     AAGAACCTTT ACGACAAGGT CCGACTACAG CTTAGGGATA ATGCAAAGGA GCTGGGTAAC
1441     GGTTGTTTCG AGTTCTATCA CAAATGTGAT AATGAATGTA TGGAAAGTGT AAGAAACGGA
1501     ACGTATGACT ACCCGCAGTA TTCAGAAGAA GCAAGATTAA AAAGAGAGGA AATAAGTGGA
1561     GTAAAATTGG AATCAATAGG AACTTACCAA ATACTGTCAA TTTATTCAAC AGTTGCGAGT
1621     TCTCTAGCAC TGGCAATCAT GGTGGCTGGT CTATCTTTGT GGATGTGCTC CAATGGGTCG
1681     TTACAATGCA GAATTTGCAT TTAA

 

Quality Control:cDNA sequencing results is available upon request.