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HA Mouse MAb

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    Influenza HA Antibody Product Information
    Immunogen:Recombinant H5N1 HA protein
    Clone ID:9F2E3F3
    Ig Type:Mouse IgG1
    Formulation:0.2 μm filtered solution in PBS
    Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H5N1 (Avian Flu) hemagglutinin extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
    Influenza HA Antibody Usage Guide
    Specificity:H5N1 (A/Anhui/1/2005) HA
    Has cross-reactivity in ELISA with
    H5N1 (A/turkey/Turkey/1/2005) HA
    H5N1 (A/chicken/India/NIV33487/2006) HA
    H5N1 (A/Hong Kong/483/97) HA
    H5N1 (A/whooper swan/mongolia/244/2005) HA
    H5N1 (A/Viet nam/1194/2004) HA
    H5N1 (A/goose/Guiyang/337/2006) HA
    H5N1 (A/Indonesia/5/2005) HA
    H5N1 (A/Cambodia/R0405050/2007) HA
    H5N1 (A/duck/Hunan/795/2002) HA
    H5N1 (A/Egypt/2321-NAMRU3/2007) HA
    H5N1 (A/Egypt/N05056/2009) HA
    H5N3 (A/duck/Hokkaido/167/2007) HA
    H5N8 (A/duck/NY/191255-59/2002) HA
    H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
    H5N1 (A/Common magpie/Hong Kong/2256/2006) HA
    No cross-reactivity in ELISA with
    H1N1 (A/California/04/2009) HA
    H1N1 (A/Brisbane/59/2007) HA
    H3N2 (A/Brisbane/10/2007) HA
    Influenza B (B/Florida/4/2006) HA
    Human cell lysate (293 cell line)
    Application:WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP, B/N

    This antibody has neutralizing activity against H5N1 (A/Anhui/1/2005) strain and H5N1 (A/bar-headed/goose/Qinghai/14/2008) strain, but no neutralizing activity against H1N1 strains through pseudotyped neutralization assay. Other H5N1 strains were not tested. The concentration of this antibody with 50% neutralization is 2 μg/ml.

    WB: 0.1-1 μg/mL

    ELISA: 0.5-1 μg/mL

    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H5N1 HA. The detection limit for H5N1 HA is approximately 2.5 ng/well.

    Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
    Other HA Antibody Products
    HA Background

    The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

    Influenza HA References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
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    Catalog: 11048-MM01-50
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    50 µg
    100 µg
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.