Influenza A H5N1 (Avian Flu) Hemagglutinin Antibody

Influenza A H5N1 (Avian Flu) HA Rabbit Monoclonal Antibody

Influenza A H5N1 HA Antibody Product Information

• Immunogen: Recombinant H5N1 HA protein
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 89
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant influenza A virus H5N1 (Avian Flu) Hemagglutinin extracellular domain.

Influenza A H5N1 HA Antibody Usage Guide

• Specificity: H5N1 (A/Anhui/1/2005) HA
• Has cross-reactivity in ELISA with

  H5N1 (A/turkey/Turkey/1/2005) HA

  H5N1 (A/Indonesia/5/2005) HA

  H5N1 (A/Viet Nam/1203/2004) HA

  H5N1 (A/chicken/India/NIV33487/2006) HA

  H5N1 (A/goose/Guiyang/337/2006) HA

  H5N1 (A/Hong Kong/483/97) HA

  H5N1 (A/Cambodia/R0405050/2007) HA

  H5N1 (A/Egypt/N05056/2009) HA

  H5N1 (A/duck/Hunan/795/2002) HA

  H5N1 (A/Egypt/2321-NAMRU3/2007) HA

  H5N3 (A/duck/Hokkaido/167/2007) HA

  H5N8 (A/duck/NY/191255-59/2002) HA

  H5N1 (A/whooper swan/Mongolia/224/2005) HA

  H5N1 (A/bar-headed goose/Qinghai/14/2008) HA

  H5N1 (A/Common magpie/Hong Kong/2256/2006) HA

• No cross-reactivity in ELISA with

  H1N1 (A/California/04/2009) HA

  H1N1 (A/California/04/2009) HA1 Sub-unit

  H1N1 (A/Brisbane/59/2007) HA

  H1N1 (A/Brevig Mission/1/1918) HA

  H5N1 (A/Anhui/1/2005) HA2 Sub-unit

  H3N2 (A/Brisbane/10/2007) HA

  Influenza B (B/Florida/4/2006) HA

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect H5N1 HA in WB
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect H5N1 HA. The detection limit for H5N1 HA is 0.16 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Influenza A H5N1 HA Antibody Background

Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species. Influenza B is largely confined to humans and is an important cause of morbidity. Influenza C infects humans, dogs and pigs, sometimes causing both severe illness and local epidemics. Influenza A is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). The notation HhNn is used to refer to the subtype comprising the hth discovered HA protein and the nth discovered NA protein. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. Subtypes are further divided into strains. Each genetically distinct virus isolate is usually considered to be a separate strain.
Influenza A virus subtype H5N1, also known as "bird flu", A(H5N1) or simply H5N1, is a subtype of the Influenza A virus which can cause illness in humans and many other animal species. H5N1 is easily transmissible between birds facilitating a potential global spread of H5N1. It is mainly spread by domestic poultry, both through the movements of infected birds and poultry products and through the use of infected poultry manure as fertilizer or feed. Humans with H5N1 have typically caught it from chickens, which were in turn infected by other poultry or waterfowl. Influenza H5N1 (A/Anhui/1/2005) virus was isolated from a specimen of tracheal aspirate. The whole genome sequencing indicated that all segments were of avian origin. The hemagglutinin receptor binding site was similar to those of other avian H5N1 viruses, and a polybasic amino acid cleavage site was present.

Related Influenza Virus Research Tools

• Influenza Virus Research Tools
• Influenza Hemagglutinin & Antibody
• Influenza Antibodies
• H5N1 Hemagglutinin & Antibody
• H5N1 Hemagglutinin Antibodies
• A/Anhui/1/2005

References

1. Li KS, et al., 2004, Nature. 430: 209-13.
2. The Writing Committee of the WHO Consultation on Human Influenza A/H5, 2005, N Engl J Med 353 (13): 1374-85.
3. Taubenberger JK, et al., 2008. Annu Rev Pathol. 3: 499-522.
4. Christophe F, et al., 2009, Science. 324:1557-1561.
5. Guan Y, et al., 2009, Revue scientifique et technique. 23(2): 453-65. 
6. Morbidity and Mortality Weekly Report. 2009-04-24. p. 400-402.