Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin Gene cDNA Clone / ORF Clone (codon optimized)

Influenza A H4N6 (A/mallard/Ohio/657/2002) cDNA Clone Datasheet

Influenza A H4N6 (A/mallard/Ohio/657/2002) cDNA Clone Product Information

• cDNA Size: 1717
• Gene Synonym: HA1, Hemagglutinin
• Source: Influenza Virus

Influenza A H4N6 (A/mallard/Ohio/657/2002) cDNA Clone Description

• cDNA Description: ORF Clone of Influenza A H4N6 (A/mallard/Ohio/657/2002) HA DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: Vector 3. Restriction site: Hind III + XhoI

Influenza A H4N6 (A/mallard/Ohio/657/2002) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Influenza A H4N6 (A/mallard/Ohio/657/2002) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Influenza A H4N6 (A/mallard/Ohio/657/2002) cDNA Clone Sequence and Quality Control

Sequence:

1        AAGCTTGCCG CCACCATGCT GAGCATTGCC ATCCTGTTCC TGCTGATTGC TGAGGGCTCC
61       AGCCAGAACT ACACAGGCAA CCCTGTGATT TGTCTGGGAC ACCATGCTGT GAGCAATGGC
121      ACAATGGTGA AGACCCTGAC AGATGACCAG ATTGAGGTGG TGACAGCCCA GGAACTGGTG
181      GAGAGCCAAC ACCTGCCTGA ACTGTGTCCA AGCCCACTGA GACTGGTGGA TGGACAAACT
241      TGTGACATTG TGAATGGAGC CCTGGGCAGC CCTGGCTGTG ACCACCTGAA TGGAGCAGAG
301      TGGGATGTGT TCATTGAGAG ACCAACAGCA GTGGACACTT GTTACCCATT TGATGTGCCT
361      GACTACCAGT CCCTGAGGAG CATCCTGGCT AACAATGGCA AGTTTGAGTT CATTGCTGAG
421      GAGTTCCAGT GGAACACAGT GAAGCAGAAT GGCAAGTCTG GAGCCTGTAA GAGGGCTAAT
481      GTGAATGACT TCTTCAACAG ACTGAACTGG CTGACCAAGT CTGATGGCAA TGCCTACCCA
541      CTCCAAAACC TGACCAAGGT GAACAATGGA GACTATGCCA GACTCTACAT CTGGGGAGTG
601      CATCACCCAA GCACAGACAC AGAACAGACC AACCTCTACA AGAACAACCC TGGCAGGGTG
661      ACAGTGAGCA CCCAGACCAG CCAGACCTCT GTGGTGCCAA ACATTGGCAG CAGACCATGG
721      GTGAGGGGAC AATCTGGCAG GATTTCCTTC TACTGGACCA TTGTGGAACC TGGAGACCTG
781      ATTGTGTTCA ACACCATTGG CAACCTGATT GCCCCAAGGG GACACTACAA ACTGAACAGC
841      CAGAAGAAGA GCACCATCCT GAACACAGCA GTGCCAATTG GCTCCTGTGT GAGCAAGTGT
901      CACACAGACA AGGGCAGCAT CAGCACCACC AAGCCATTCC AGAACATCAG CAGGATTAGC
961      ATTGGAGACT GTCCAAAATA TGTGAAGCAG GGCTCCCTGA AACTGGCTAC AGGGATGAGG
1021     AACATCCCTG AGAAGGCTAC CAGGGGACTG TTTGGAGCCA TTGCTGGCTT CATTGAGAAT
1081     GGCTGGCAGG GACTGATTGA TGGCTGGTAT GGCTTCAGAC ACCAGAATGC TGAGGGCACA
1141     GGCACAGCAG CAGACCTGAA AAGCACCCAG GCTGCCATTG ACCAGATAAA TGGCAAACTG
1201     AACAGACTGA TTGAGAAGAC CAATGAGAAA TACCACCAGA TTGAGAAGGA GTTTGAACAG
1261     GTGGAGGGCA GGATTCAGGA CTTGGAGAAA TATGTGGAGG ACACCAAGAT TGACCTGTGG
1321     TCCTACAATG CTGAACTGCT GGTGGCTTTG GAGAACCAAC ACACCATTGA TGTGACAGAC
1381     TCTGAGATGA ACAAACTGTT TGAGAGGGTG AGGAGACAAC TTAGGGAGAA TGCTGAGGAC
1441     AAGGGCAATG GCTGTTTTGA GATTTTCCAC CAGTGTGACA ACTCCTGTAT TGAGAGCATC
1501     AGGAATGGCA CCTATGACCA TGACATCTAC AGGGATGAGG CTATCAACAA CAGGTTCCAG
1561     ATTCAGGGAG TGAAACTGAC CCAGGGCTAC AAGGACATCA TCCTGTGGAT TTCCTTCAGC
1621     ATCTCCTGTT TCCTGCTGGT GGCTCTGCTG CTGGCTTTCA TCCTGTGGGC TTGTCAGAAT
1681     GGCAACATCA GGTGTCAGAT TTGTATTTAA ATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.